Library Science

In Hematologic Malignancies: Methods and Techniques, a panel of acknowledged experts review many of the key molecular methods used for the diagnosis and subsequent management of hematologic malignancies. These clinically relevant techniques range from routine test procedures to highly sophisticated methods currently offered only by specialized reference laboratories, and fall into five major groups: cytogenetics, polymerase chain reaction, flow cytometry, cytochemistry and immunochemistry, and apoptosis and cytokine receptors. Serving both clinical and experimental needs, Hematologic Malignancies: Methods and Techniques provides an array of powerful tools that will guide clinicians- especially hematologists, oncologists, and pathologists-to better diagnose and manage their patients with hematologic malignancies, and enable researchers to assess the anticancer effect of agents that impact cancer cells at the molecular level.

Authors: Faguet, Guy B.

Table of Contents

1. Cytogenetics Analysis (pp. 3-18)
   Sandberg, Avery A.; Chen, Zhong
2. FISH Analysis (pp. 19-42)
   Sandberg, Avery A.; Chen, Zhong
3. Comparative Genomic Hybridization for the Analysis of Leukemias and Lymphomas (pp. 43-64)
   Baudis, Michael; Bentz, Martin
4. Spectral Karyotyping (SKY) of Hematologic Malignancies (pp. 65-79)
   Hilgenfeld, Eva; Padilla-Nash, Hesed; Haas, Oskar A.; Serve, Hubert; Schrock, Evelin; Ried, Thomas
5. Molecular Genetic Methods in Diagnosis and Treatment (pp. 83-116)
   Ward, Pamela; Kinniburgh, Alan
6. Detecting Monoclonal Immunoglobulin and T-Cell Receptor Gene Rearrangements in B- and T-Cell Malignancies by Polymerase Chain Reaction (pp. 117-132)
   Sykes, Pamela J.
7. Quantifying Residual Leukemia by ??°Clone-Specific??² Polymerase Chain Reaction (pp. 133-156)
   Brisco, Michael J.
8. NPM-ALK Reverse Transcriptase-Polymerase Chain Reaction for Detecting the t(2;5) of Non-Hodgkin???s Lymphoma (pp. 157-176)
   Shurtleff, Sheila A.; Downing, James R.; Morris, Stephan W.
9. Diagnostic Flow Cytometry in Hematologic Malignancies (pp. 179-215)
   Marti, Gerald E.; Stetler-Stevenson, Maryalice; Fleisher, Thomas
10. Flow Cytometric Analysis of Hematologic Neoplasia (pp. 217-230)
    Braylan, Raul C.; Anderson, John B.
11. In Situ Hybridization in Flow Cytometry (pp. 231-253)
    Mosiman, Victoria L.; Goolsby, Charles L.
12. Quantitative Fluorescence Cytometry (pp. 255-273)
    Vogt, Robert F.; Schwartz, Abe; Marti, Gerald E.; Whitfield, Wanda E.; Henderson, L. Omar
13. Methods in Leukocyte Cytochemistry (pp. 277-300)
    Hanly, Mark G.
14. Immunohistochemistry in Leukemias and Lymphomas (pp. 301-319)
    Jaramillo, Marina; Rangel, Catherine; Grogan, Thomas
15. Apoptosis: Molecular Regulation of Cell Death and Hematologic Malignancies (pp. 323-338)
    Chiarugi, Vincenzo; Cinelli, Marina; Magnelli, Lucia; Sbarba, Persio Dello
16. Cytokine Receptors: Qualitative and Quantitative Analysis (pp. 339-351)
    Zola, Heddy

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