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Helicobacter pylori Protocols

13th December 2006

Helicobacter pylori Protocols

posted in BioLibrary, Library, Molecular Medicine |
Helicobacter pylori Protocols offers an outstanding collection of state-of-the-art protocols for the identification and molecular manipulation of H. pylori. The authoritative contributors supply detailed and readily reproducible protocols for the culturing of H. pylori, for the isolation and restriction endonuclease digestion of H. pylori chromosomal DNA, and for the transformation and insertional mutagenesis of H. pylori. They also provide molecular epidemiological techniques, including ribotyping, PCR-RFLP, and RAPD-PCR. These procedures have been developed by leading practitioners to solve the difficult technical problems created by the application of the powerful bacterial genetic and molecular cloning techniques to H. pylori.Ed. Clayton, Christopher L. ; Mobley, Harry L. T.

Table of Contents

    1. Introduction: Medical Significance of H. pylori (pp. 1-6)
      Blaser, Martin J.
    2. Detection of H. pyhi Infection by Biopsy Urease, Histology, and Culture (pp. 7-18)
      Goodwin, Stewart
    3. Serology and Urea Breath Test in the Diagnosis of H. pylori Infection (pp. 19-30)
      Hawtin, Peter R.
    4. The Detection of H. pylori by the Polymerase Chain Reaction (pp. 31-36)
      Mapstone, Nicholas P.
    5. PCR for the Detection of H. pylori in Gastric Juice Aspirates and Environmental Water Samples (pp. 37-40)
      Fritz, Stephen B.; Westblom, T. Ulf
    6. In vitro Susceptibility Testing of H. pylori (pp. 41-52)
      McLaren, Alistair
    7. Defined Media for H. pylori (pp. 53-62)
      Reynolds, David J.
    8. Studies of Respiration in H. pylori (pp. 63-68)
      Miles, Roger J.; Chang, Huai-Tzu
    9. Use of NMR to Study H. pylori Metabolism (pp. 69-80)
      Chalk, Peter A.; Roberts, Andrew D.; Davison, Alistair A.; Kelly, David J.; White, Peter J.
    10. Isolation of H. py/oriGenomic DNA and Restriction Analysis (pp. 81-88)
      Owen, Robert J.; Bickley, Jane
    11. Ribosomal RNA Gene Restriction Pattern Analysis (Ribotyping) of H. pylori (pp. 89-98)
      Tee, Wee
    12. Differentiation of H. pylori Strains Using PCR RFLP (pp. 99-106)
      Mobley, Harry L. T.; Foxall, Paul A.
    13. PCR-DNA Sequence Typing (pp. 107-116)
      Labigne, Agnes
    14. H. pylori DNA Fingerprinting Using the Arbitrarily Primed PCR (AP-PCR) or Random Amplified Polymorphic DNA (RAPD) Method (pp. 117-132)
      Berg, Douglas E.; Lelwala-Guruge, Janaki; Incecik, Engin T.; Srivastava, Kalpana; Akopyants, Natalia S.
    15. Molecular Methods for Detecting Ulcerogenic Strains of H. pylori (pp. 133-144)
      Atherton, John C.
    16. H. pylori DNA Transformation by Natural Competence and Electroporation (pp. 145-152)
      Ge, Zhongming; Taylor, Diane E.
    17. Random Mutagenesis of the H. pylori Genome (pp. 153-164)
      Labigne, Agnes
    18. Construction of a Genomic Map of H. pylori by Pulsed-Field Gel Electrophoresis (PFGE) (pp. 165-176)
      Chang, Nicholas; Jiang, Qin; Taylor, Diane E.
    19. Molecular Characterization of H. pylori Surface Antigens (pp. 177-190)
      Doig, Peter; O?Toole, Paul W.; Trust, Trevor J.
    20. Isolation and Characterization of Porins from H. pylori (pp. 191-204)
      Hancock, Robert E. W.; Exner, Maurice
    21. Methods for the Identification of H. pylori Host Receptors (pp. 205-224)
      Bore, Thomas; Wadstrom, Torkel; Normark, Staffan; Gordon, Jeffrey I.; Falk, Per G.
    22. Host Responses to H. pylori: Molecular Analysis of Cytokine Gene Expression (pp. 225-234)
      Farmery, Susan M.; Crabtree, Jean E.
    23. Nonprimate Animal Models of H. pylori Infection (pp. 235-252)
      McColm, Andrew A.
    24. The Nonhuman Primate Model for H. pylori Infection (pp. 253-270)
      Dubois, Andre; Berg, Douglas E.

This entry was posted on Wednesday, December 13th, 2006 at 12:57 pm and is filed under BioLibrary, Library, Molecular Medicine. You can follow any responses to this entry through the RSS 2.0 feed. You can leave a response, or trackback from your own site.

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