Library Science

Genetic recombination is any process in which DNA sequences interact and undergo a transfer of information, producing new “recombinant” sequences that contain information from each of the original molecules. This book presents leading peer-reviewed protocols to carry out recombinant investigations in the lab. Part I provides approaches and model systems for studying several aspects of recombination in a variety of eukaryotic organisms and in mammalian parasites. Part II describes approaches for using recombination as a reporter of genomic instability in lower and higher eukaryotes. Part III discusses various methods and approaches for targeted genomic manipulation in higher and lower eukaryotes, while the final part presents biochemical analyses useful for furthering the understanding of recombination mechanisms, thus making this an invaluable book for both the novice and the established researcher in the field of recombination.

Authors: Waldman, Alan S.

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Table of Contents

1. Determination of Mitotic Recombination Rates by Fluctuation Analysis in Saccharomyces cerevisiae (pp. 003-012)
   Spell, Rachelle Miller; Jinks-Robertson, Sue
2. Determination of Intrachromosomal Recombination Rates in Cultured Mammalian Cells (pp. 013-024)
   Smith, Jason A.; Waldman, Alan S.
3. Intrachromosomal Homologous Recombination in Arabidopsis thaliana (pp. 025-034)
   Schmidt-Puchta, Waltraud; Orel, Nadiya; Kyryk, Anzhela; Puchta, Holger
4. Analysis of Recombinational Repair of DNA Double-Strand Breaks in Mammalian Cells With I-SceI Nuclease (pp. 035-052)
   Nickoloff, Jac A.; Brenneman, Mark A.
5. Transformation of Monomorphic and Pleomorphic Trypanosoma brucei (pp. 053-086)
   McCulloch, Richard; Vassella, Erik; Burton, Peter; Boshart, Michael; Barry, J. David
6. Forward Genetic Screens for Meiotic and Mitotic Recombination-Defective Mutants in Mice (pp. 087-108)
   Reinholdt, Laura; Ashley, Terry; Schimenti, John; Shima, Naoko
7. Detecting Carcinogens With the Yeast DEL Assay (pp. 111-124)
   Brennan, Richard J.; Schiestl, Robert H.
8. In Vivo DNA Deletion Assay to Detect Environmental and Genetic Predisposition to Cancer (pp. 125-140)
   Reliene, Ramune; Bishop, Alexander J. R.; Aubrecht, Jiri; Schiestl, Robert H.
9. Gene Targeting at the Chromosomal Immunoglobulin Locus: A Model System for the Study of Mammalian Homologous Recombination Mechanisms (pp. 143-156)
   Baker, Mark D.
10. DNA Fragment Transplacement in Saccharomyces cerevisiae: Some Genetic Considerations (pp. 157-172)
    Manthey, Glenn M.; Navarro, Michelle S.; Bailis, Adam M.
11. Targeted Gene Modification Using Triplex-Forming Oligonucleotides (pp. 173-194)
    Kuan, Jean Y.; Glazer, Peter M.
12. Using Nucleases to Stimulate Homologous Recombination (pp. 195-208)
    Carroll, Dana
13. Enhancement of In Vivo Targeted Nucleotide Exchange by Nonspecific Carrier DNA (pp. 209-220)
    Maguire, Katie K.; Kmiec, Eric B.
14. Chromatin Immunoprecipitation to Investigate Protein–DNA Interactions During Genetic Recombination (pp. 223-238)
    Goldfarb, Tamara; Alani, Eric
15. Holliday Junction Branch Migration and Resolution Assays (pp. 239-254)
    Constantinou, Angelos; West, Stephen C.

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