Library Science

Thirty protocols by leading researchers describe in detail all the essential molecular methods for working with gene transfer systems, along with the methods for gene transfer to specific tissue types either in vivo or ex vivo. The easily reproducible methods range from those for specific viral and nonviral for both genetic and acquired diseases, to those concerned with gene delivery to particular tissues. Methods for applying specific therapeutic systems, such as ribozymes and tumor supressor genes for the treatment of AIDS and cancer, are also included in this authoritative collection.

Ed. Robbins, Paul D.

Table of Contents

1. Methods for Gene Transfer Using DNA-Adenovirus Conjugates (pp. 1-24)
   Curiel, David T.
2. Methods for the Construction and Propagation of Recombinant Adeno-Associated Virus Vectors (pp. 25-40)
   Bartlett, Jeffrey S.; Samulski, Richard J.
3. Generation of High-Titer, Helper-Free Retroviruses by Transient Transfection (pp. 41-58)
   Pear, Warren S.; Scott, Martin L.; Nolan, Garry P.
4. Methods for the Construction of Retroviral Vectors and the Generation of High-Titer Producers (pp. 59-78)
   Riviere, lsabelle; Sadelain, Michel
5. Development of Replication-Defective Herpes Simplex Virus Vectors (pp. 79-102)
   Krisky, David; Marconi, Peggy; Goins, William F.; Glorioso, Joseph C.
6. Methods for the Use of Poliovirus Vectors for Gene Delivery (pp. 103-116)
   Morrow, Casey D.; Ansardi, David C.; Porter, Donna C.
7. Methods for the Construction of Human Papillomavirus Vectors (pp. 117-126)
   Khan, Saleem A.; Sverdrup, Francis M.
8. Methods for Liposome-Mediated Gene Transfer to the Arterial Wall (pp. 127-134)
   Yang, Elizabeth G. Nabel Zhi-yong; San, Hong; Carr, Dianne P.; Nabel, Gary J.
9. Methods for Targeted Gene Transfer to Liver Using DNA-Protein Complexes (pp. 135-152)
   Findeis, Mark A.; Wu, Catherine H.; Wu, George Y.
10. Methods for the Use of Retroviral Vectors for Transfer of the CFTR Gene to Airway Epithelium (pp. 153-168)
    Olsen, John C.; Johnson, Larry G.; Yankaskas, James R.
11. Methods for Adenowirus-Mediated Gene Transfer to Airway Epithelium (pp. 169-184)
    Engelhardt, John F.
12. Methods for Retrovirus-Mediated Gene Transfer to Fetal Lung (pp. 185-194)
    Pitt, Bruce R.; Schwarz, Margaret A.; Bland, Richard D.
13. In Situ Retrovirus-Mediated Gene Transfer into the Liver (pp. 195-204)
    Ferry, Nicolas; Bnanchereau, Sophie; Heard, Jean-Michel; Danos, Olivier
14. Methods for Delivery of Genes to Hepatocytes In Vivo Using Recombinant Adenovirus Vectors (pp. 205-212)
    Barr, Darlene; Kay, Mark A.
15. Methods for Producing High-Titer, Pantropic Retroviral Vectors for Gene Transfer into Leukemic T-Cellls (pp. 213-222)
    Burns, Jane C.; Yee, Jiing-Kuan; Yu, Alice L.
16. Methods for Efficient Retrovirus-Mediated Gene Transfer to Mouse Hematopoietic Stem Cells (pp. 223-240)
    Belmont, John W.; Jurecic, Roland
17. Methods for Retrovirus-Mediated Gene Transfer into Primary T-Lymphocytes (pp. 241-248)
    Sadelain, Michel
18. Methods for Retrovirus-Mediated Gene Transfer to CD34+-Enriched Cells (pp. 249-264)
    Bahnson, Alfred B.; Nimgaonkar, Maya; Ball, Edward D.; Barranger, John A.
19. Methods for the Use of Genetically Modified Keratinocytes in Gene Therapy (pp. 265-280)
    Eming, Sabine A.; Morgan, Jeffrey R.
20. Methods for Particle-Mediated Gene Transfer into Skin (pp. 281-296)
    Yang, Ning-Sun; McCabe, Dennis E.; Swain, William F.
21. Methods for Liposome-Mediated Gene Transfer to Tumor Cells In Vivo (pp. 297-306)
    Nabel, Gary J.; Yang, Zhi-yong; Nabel, Elizabeth G.
22. Methods for Retrovirus-Mediated Gene Transfer to Tumor Cells (pp. 307-328)
    Jaffee, Elizabeth M.
23. Cationic Liposome-Mediated Gene Transfer to Tumor Cells In Vitro and In Vivo (pp. 329-338)
    Son, Kyonghee; Sorgi, Frank; Gao, Xiang; Huang, Leaf
24. Methods for the Use of Cytokine Gene-Modified Tumor Cells in Immunotherapy of Cancer (pp. 339-348)
    Qin, Zhihai; Blankenstein, Thomas
25. Methods for Generation of Genetically Modified Fibroblasts for Immunotherapy of Cancer (pp. 349-356)
    Elder, Elaine M.; Lotze, Michael T.; Whiteside, Theresa L.
26. Methods for Gene Transfer to Synovium (pp. 357-368)
    Kang, Richard; Robbins, Paul D.; Evans, Christopher H.
27. Methods for Adenovirus-Mediated Gene Transfer to Synovium In Vivo (pp. 369-374)
    Roessler, Blake J.
28. Methods for the Use of Stromal Cells for Therapeutic Gene Therapy (pp. 375-390)
    Greenberger, Joel S.
29. Suppression of the Human Carcinoma Phenotype by an Antioncogene Ribozyme (pp. 391-402)
    Shitara, Toshiya; Scanlon, Kevin J.
30. Methods for Cancer Gene Therapy Using Tumor Suppressor Genes (pp. 403-418)
    Zhang, Wei-Wei; Roth, Jack A.

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