Library Science

In this fully updated and revised 2nd edition of Gene Therapy Protocols, leading experts from academic and industrial laboratories around the world detail their most effective viral and nonviral methods of gene transfer, as well as discuss their applications in different organ systems. These methods range from those in which new molecular conjugates show great promise for targeting gene transfer and regulating transgene expression, to those used in such exciting applications as the delivery of therapeutic proteins, vaccination, and tissue engineering. Up-to-date and highly practical, Gene Therapy Protocols, 2nd Edition, offers a rich compilation of the revolutionary advances that have recently occurred in gene transfer technology, with each article providing proven the step-by-step laboratory procedures that enable its successful therapeutic application.

Authors: Morgan, Jeffrey R.

Table of Contents

1. Poly-L-Lysine-Based Gene Delivery Systems: Synthesis, Purification, and Application (pp. 001-013)
   Lollo, Charles P; Banaszczyk, Mariusz G; Mullen, Patricia M; Coffin, Christopher C; Wu, Dongpei; Carlo, Alison T; Bassett, Donna L; Gouveia, Erin K; Carlo, Dennis J
2. Targeted Gene Transfer to Liver Using Protein-DNA Complexes (pp. 015-023)
   Wu, Catherine H; Walton, Cherie M; Wu, George Y
3. Receptor-Directed Molecular Conjugates for Gene Transfer (pp. 025-048)
   Ziady, Assem G; Davis, Pamela B
4. Gene Transfer into Muscle by Electroporation In Vivo (pp. 049-062)
   Miyazaki, Jun-ichi; Aihara, Hiroyuki
5. Viral Liposomes: Preparation and Use (pp. 063-072)
   Kaneda, Yasufumi
6. LPD Nanoparticles-Novel Nonviral Vector for Efficient Gene Delivery (pp. 073-081)
   Tan, Yadi; Whitmore, Mark; Li, Song; Frederik, Peter; Huang, Leaf
7. Solvoplex Synthetic Vector for Intrapulmonary Gene Delivery: Preparation and Use (pp. 083-094)
   Schughart, Klaus; Rasmussen, Ulla B
8. Gene Correction Frequency by Chimeric RNA-DNA Oligonucleotide Using Nuclear Extracts (pp. 095-107)
   Igoucheva, Olga A; Yoon, Kyonggeun
9. Regulated Expression of Plasmid-Based Gene Therapies (pp. 109-122)
   Abruzzese, Ronald V; MacLaughlin, Fiona C; Smith, Louis C; Nordstrom, Jeffrey L
10. Photochemical Transfection: Light-Induced, Site-Directed Gene Delivery (pp. 123-135)
    Prasmickaite, Lina; Hogset, Anders; Berg, Kristian
11. Direct Gene Transfer and Vaccination Via Skin Transfection Using a Gene Gun (pp. 137-147)
    Kuo, Chia-Feng; Wang, Jeng Hwan; Yang, Ning-Sun
12. Preparation of Pseudotyped Retroviral Vector (pp. 149-159)
    Yu, Hong
13. Quantitative Measurement of the Concentration of Active Recombinant Retrovirus (pp. 161-172)
    Andreadis, Stelios T; Morgan, Jeffrey R
14. Minigene-Containing Retroviral Vectors Using an Alphavirus/Retrovirus Hybrid Vector System: Production and Use (pp. 173-186)
    Wahlfors, Jarmo; Morgan, Richard A
15. Retrovirus-Mediated Gene Transfer to Human Hematopoietic Stem Cells (pp. 187-202)
    Swaney, William P; Novelli, Enrico M; Bahnson, Alfred B; Barranger, John A
16. Genetically Modified Skin Substitutes: Preparation and Use (pp. 203-217)
    Hamoen, Karen E; Erdag, Gulsun; Cusick, Jennifer L; Rakhorst, Hinne A; Morgan, Jeffrey R
17. Bioartificial Muscles in Gene Therapy (pp. 219-231)
    Powell, Courtney; Shansky, Janet; Del Tatto, Michael; Vandenburgh, Herman H
18. Cytokine Gene-Modified Cell-Based Cancer Vaccines (pp. 233-257)
    Reilly, R Todd; Machiels, Jean-Pascal H; Emens, Leisha A; Jaffee, Elizabeth M
19. HIV-Based Vectors: Preparation and Use (pp. 259-274)
    Follenzi, Antonia; Naldini, Luigi
20. Packaging Cell System for Lentivirus Vectors: Preparation and Use (pp. 275-302)
    Srinivasakumar, Narasimhachar
21. Lentiviral Vectors: Preparation and Use (pp. 303-318)
    Chang, Lung-Ji; Zaiss, Anne-Kathrin
22. Simian Foamy Virus Vectors: Preparation and Use (pp. 319-333)
    Park, Jeonghae; Mergia, Ayalew
23. Recombinant Feline Immunodeficiency Virus Vectors: Preparation and Use (pp. 335-350)
    Curran, Michael A; Nolan, Garry P
24. Lentivirus Vector Based on Simian Immunodeficiency Virus: Development and Use (pp. 351-360)
    Uberla, Klaus
25. Cytoplasmic RNA Vector Derived from Nontransmissible Sendai Virus: Production and Use (pp. 361-370)
    Iida, Akihiro; Hasegawa, Mamoru
26. Preparation of Helper-Dependent Adenoviral Vectors (pp. 371-388)
    Ng, Philip; Parks, Robin J; Graham, Frank L
27. Construction of First-Generation Adenoviral Vectors (pp. 389-414)
    Ng, Philip; Graham, Frank L
28. Preparation of Ovine Adenovirus Vectors (pp. 415-426)
    Loser, Peter; Kumin, Daniel; Hillgenberg, Moritz; Both, Gerald W; Hofmann, Christian
29. Highly Purified Recombinant Adeno-Associated Virus Vectors: Preparation and Quantitation (pp. 427-443)
    Schnepp, Bruce; Clark, K Reed
30. High-Titer Stocks of Adeno-Associated Virus from Replicating Amplicons and Herpes Vectors (pp. 445-460)
    Mistry, Ajay R; De Alwis, Mahesh; Feudner, Elisabeth; Ali, Robin R; Thrasher, Adrian J
31. Herpes Simplex Virus/Adeno-Associated Virus Hybrid Vectors for Gene Transfer to Neurons: Preparation and Use (pp. 461-479)
    Costantini, Lauren C; Fraefel, Cornel; Breakefield, Xandra O; Isacson, Ole
32. Development of Replication-Defective Herpes Simplex Virus Vectors (pp. 481-507)
    Goins, William F; Krisky, David M; Wolfe, Darren P; Fink, David J; Glorioso, Joseph C
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