Library Science

Highly experienced researchers describe in step-by-step detail methods that have proven most useful in delivering genes to mammalian cells. Volume 1: Nonviral Gene Transfer Techniques focuses on gene delivery by a variety of chemical and physical methods, including ultrasound, biolistics, peptides, PNA clamps, liposomes, microinjection, electroporation, particle bombardment, dendrimers, and hydrodynamics. An accompanying volume, Volume 2: Viral Gene Transfer Techniques, details procedures for delivering genes to cells in vitro and in vivo, including the use of lentiviral vectors.

Ed. Heiser, William C.

 gene-delivery-to-mammalian-cells-volume-1-nonviral-gene-transfer-techniques.pdf

Table of Contents

1. Chemical Methods for DNA Delivery: An Overview (pp. 3-24)
   Liu, Dexi; Chiao, Evelyn F.; Tian, Hui
2. Gene Transfer into Mammalian Cells Using Calcium Phosphate and DEAE-Dextran (pp. 25-32)
   Pari, Gregory S.; Xu, Yiyang
3. DNA Delivery to Cells in Culture Using Peptides (pp. 33-52)
   Zhang, Lei; Ambulos, Nicholas; Mixson, A. James
4. DNA Delivery to Cells in Culture Using PNA Clamps (pp. 53-66)
   Giorgio, Todd D.; Wyatt, Shelby K.
5. Dendrimer-Mediated Cell Transfection In Vitro (pp. 67-82)
   Baker, James R.; Bielinska, Anna U.; Kukowska-Latallo, Jolanta F.
6. DNA Delivery to Cells in Culture Using Cationic Liposomes (pp. 83-94)
   Wyatt, Shelby K.; Giorgio, Todd D.
7. Formulation of Synthetic Gene Delivery Vectors for Transduction of the Airway Epithelium (pp. 95-114)
   Marshall, John; Yew, Nelson S.; Cheng, Seng H.
8. Cationic Liposome-Mediated DNA Delivery to the Lung Endothelium (pp. 115-124)
   Song, Young K.; Zhang, Guisheng; Liu, Dexi
9. Delivery of DNA to Tumor Cells Using Cationic Liposomes (pp. 125-136)
   Yan, Duen-Hwa; Spohn, Bill; Hung, Mien-Chie
10. Delivery of Transposon DNA to Lungs of Mice Using Polyethyleneimine-DNA Complexes (pp. 137-144)
    Belur, Lalitha R.; McIvor, R. Scott
11. Gene Delivery Using Physical Methods: An Overview (pp. 147-166)
    Chou, Te-hui W.; Biswas, Subhabrata; Lu, Shan
12. Gene Delivery to Mammalian Cells by Microinjection (pp. 167-174)
    King, Robert
13. Delivery of DNA to Cells in Culture Using Particle Bombardment (pp. 175-184)
    Heiser, William C.
14. Delivery of DNA to Skin by Particle Bombardment (pp. 185-196)
    Wang, Shixia; Joshi, Swati; Lu, Shan
15. Biolistic Transfection of Cultured Organotypic Brain Slices (pp. 197-206)
    McAllister, A. Kimberley
16. Efficient Electroporation of Mammalian Cells in Culture (pp. 207-214)
    Barry, Peter A.
17. Delivery of DNA to Skin by Electroporation (pp. 215-226)
    Dujardin, Nathalie; Preat, Veronique
18. In Vivo DNA Electrotransfer in Skeletal Muscle (pp. 227-236)
    Sanz, Guenhael; Satkauskas, Saulius; Mir, Lluis M.
19. Electrically Mediated Plasmid DNA Delivery to Solid Tumors In Vivo (pp. 237-244)
    Jaroszeski, Mark J.; Heller, Loree C.; Gilbert, Richard; Heller, Richard
20. Hydrodynamic Delivery of DNA (pp. 245-250)
    Knapp, Joseph E.; Liu, Dexi
21. Naked DNA Gene Transfer in Mammalian Cells (pp. 251-264)
    Zhang, Guofeng; Budker, Vladimir G.; Ludtke, James J.; Wolff, Jon A.
22. Microparticle Delivery of Plasmid DNA to Mammalian Cells (pp. 265-286)
    Hedley, Mary Lynne; Barman, Shikha P.
23. DNA Delivery to Cells in Culture Using Ultrasound (pp. 287-292)
    McCreery, Thomas P.; Sweitzer, Robert H.; Unger, Evan C.
24. DNA Delivery to Cells In Vivo by Ultrasound (pp. 293-298)
    McCreery, Thomas P.; Sweitzer, Robert H.; Unger, Evan C.; Sullivan, Sean

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