Library Science

Electroporation Protocols for Microorganisms is the first complete guide to the electroporation of nearly all microorganisms of importance used in biological and biomedical research. It includes reproducible protocols for diverse bacterial, fungal, and protist species-many of which are important in human disease-as well as literature references to electroporation protocols for related species. The contributors also discuss electroporation theory and instrumentation, making it possible to develop new protocols or modify existing ones, and they provide extensive details about culturing and storing many species in a manner designed to optimize electroporation efficiency. Electroporation Protocols for Microorganisms is an indispensable resource for molecular geneticists working directly with microorganisms and for those who employ microorganisms to prepare materials for later introduction into higher organisms, such as plants and animals. Two companion volumes will follow: Plant Cell Electroporation and Electrofusion Protocols and Animal Cell Electroporation and Electrofusion Protocols.

Authors: Nickoloff, Jac A.

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Table of Contents

1. Electroporation Theory: Concepts and Mechanisms (pp. 1-26)
   Weaver, James C.
2. Instrumentation (pp. 27-46)
   Hofmann, Gunter A.
3. Direct Plasmid Transfer Between Bacterial Species and Electrocuring (pp. 47-54)
   Withers, Helen L.
4. Transfer of Episomal and Integrated Plasmids from Saccharomyces cerevisiae to Escherichia coli by Electroporation (pp. 55-66)
   Gunn, Laura; Whelden, Jennifer; Nickoloff, Jac A.
5. Production of cDNA Libraries by Electroporation (pp. 67-80)
   Gruber, Christian E.
6. Electroporation of RNA into Saccharomyces cerevisiae (pp. 81-92)
   Gallie, Daniel R.
7. Electrofusion of Yeast Protoplasts (pp. 93-104)
   Weber, Herbert; Berg, Hermann
8. Escherichia coli Electrotransformation (pp. 105-114)
   Miller, Elizabeth M.; Nickoloff, Jac A.
9. Electrotransformation in Salmonella (pp. 115-124)
   Sanderson, Kenneth E.; MacLachlan, P. Ronald; Hessel, Andrew
10. Electrotransformation of Pseudomonas (pp. 125-134)
    Dennis, Jonathan J.; Sokol, Pamela A.
11. Electroporation of Xanthomonas (pp. 135-142)
    White, Teresa J.; Gonzalez, Carlos E.
12. Transformation of Brucella Species with Suicide and Broad Host-Range Plasmids (pp. 143-148)
    McQuiston, John R.; Schurig, Gerhardt G.; Sriranganathan, Nammalwar; Boyle, Stephen M.
13. Electroporation of Francisella tularensis (pp. 149-154)
    Baron, Gerald S.; Myltseva, Svetlana V.; Nano, Francis E.
14. A Simple and Rapid Method for Transformation of Vibrio Species by Electroporation (pp. 155-160)
    Hamashima, Hajime; Iwasaki, Makoto; Arai, Taketoshi
15. Genetic Transformation of Bacteroides spp. Using Electroporation (pp. 161-170)
    Smith, C. Jeffrey
16. Electrotransformation of Agrobacterium (pp. 171-178)
    Lin, Jhy-Jhu
17. Electroporation of Helicobacter pylori (pp. 179-184)
    Segal, Ellyn D.
18. Electrotransformation of Streptococci (pp. 185-194)
    McLaughlin, Robert E.; Ferretti, Joseph J.
19. Transformation of Lactococcus by Electroporation (pp. 195-200)
    Holo, Helge; Nes, Ingolf F.
20. Transformation of Lactobacillus by Electroporation (pp. 201-208)
    Aukrust, Thea W.; Brurberg, May B.; Nes, Ingolf F.
21. Electrotransformation of Staphylococci (pp. 209-216)
    Lee, Jean C.
22. Electroporation and Efficient Transformation of Enterococcus faecalis Grown in High Concentrations of Glycine (pp. 217-226)
    Shepard, Brett D.; Gilmore, Michael S.
23. Introduction of Recombinant DNA into Clostridium spp. (pp. 227-236)
    Phillips-Jones, Mary K.
24. Electroporation of Mycobacteria (pp. 237-252)
    Parish, T.; Stoker, N. G.
25. Electrotransformation of the Spirochete Borrelia burgdorferi (pp. 253-260)
    Samuels, D. Scott
26. Yeast Transformation and the Preparation of Frozen Spheroplasts for Electroporation (pp. 261-268)
    Stowers, Lisa; Gautsch, James; Dana, Richard; Hoekstra, Merl F.
27. Ten-Minute Electrotransformation of Saccharomyces cerevisiae (pp. 269-272)
    Grey, Martin; Brendel, Martin
28. Electroporation of Schizosaccharomyces pombe (pp. 273-278)
    Hood, Mark T.; Stachow, C. S.
29. Gene Transfer by Electroporation of Filamentous Fungi (pp. 279-290)
    Kapoor, M.
30. Transformation of Candida maltosa by Electroporation (pp. 291-302)
    Becher, Dietmar; Oliver, Stephen G.
31. Electroporation of Physarum polycephalum (pp. 303-320)
    Burland, Timothy G.; Bailey, Juliet
32. Electroporation of Dictyostelium discoideum (pp. 321-330)
    Knecht, David; Pang, Ka Ming
33. Gene Transfer by Electroporation in Tetrahymena (pp. 331-348)
    Gaertig, Jacek; Gorovsky, Martin A.
34. Transfection of the African and American Trypanosomes (pp. 349-360)
    Kelly, John M.; Taylor, Martin C.; Rudenko, Gloria; Blundell, Pat A.
35. Electroporation in Giardia lamblia (pp. 361-368)
    Wang, A. L.; Sepp, Tiina; Wang, C. C.

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