Library Science

A multidisciplinary panel of leading experimentalists details the key protocols for the diagnosis and study of the pathogenesis of Shiga toxin-producing Escherichia coli (STEC) and its isolated cytotoxin (Stx). Described in step-by-step detail, these readily reproducible techniques range from those for the diagnosis and detection of STEC bacteria in patient and animal samples, to those for studying the cellular microbiology of STEC infections, especially host???pathogen interactions and the hemolysin of STEC. There are also protocols for studying the details of Shiga toxin (Stx) biology-from the purification of the toxin to the effects of Stx on various host cell functions-and for exploring STEC-mediated disease in various animal models.

Authors: Ed. Philpott, Dana ; Ebel, Frank

Table of Contents

1. The Medical Significance of Shiga Toxin-Producing Escherichia coli Infections: An Overview (pp. 01-07)
   Karmali, Mohamed A.
2. Methods for Detection of STEC in Humans: An Overview (pp. 09-26)
   Paton, James C.; Paton, Adrienne W.
3. Serological Methods for the Detection of STEC Infections (pp. 27-44)
   Bitzan, Martin; Karch, Helge
4. Detection and Characterization of STEC in Stool Samples Using PCR (pp. 45-54)
   Paton, Adrienne W.; Paton, James C.
5. Molecular Typing Methods for STEC (pp. 55-66)
   Watanabe, Haruo; Terajima, Jun; Izumiya, Hidemasa; Iyoda, Sunao
6. STEC in the Food Chain: Methods for Detection of STEC in Food Samples (pp. 67-74)
   Bulte, Michael
7. STEC as a Veterinary Problem: Diagnostics and Prophylaxis in Animals (pp. 75-90)
   Wieler, Lothar H.; Bauerfeind, Rolf
8. Cellular Microbiology of STEC Infections: An Overview (pp. 91-98)
   Ebel, Frank; Philpott, Dana
9. Analysis of Pathogenicity Islands of STEC (pp. 99-112)
   Oelschlaeger, Tobias A.; Dobrindt, Ulrich; Janke, Britta; Middendorf, Barbara; Karch, Helge; Hacker, Jorg
10. Generation of Isogenic Deletion Mutants of STEC (pp. 113-124)
    Djafari, Soudabeh; Hauf, Nadja D.; Tyczka, Judith F.
11. Generation of Monoclonal Antibodies Against Secreted Proteins of STEC (pp. 125-136)
    Niebuhr, Kirsten; Ebel, Frank
12. Microscopic Methods to Study STEC: Analysis of the Attaching and Effacing Process (pp. 137-150)
    Knutton, Stuart
13. Detection and Characterization of EHEC-Hemolysin (pp. 151-164)
    Schmidt, Herbert; Benz, Roland
14. Shiga Toxin Receptor Glycolipid Binding: Pathology and Utility (pp. 165-186)
    Lingwood, Clifford A.
15. Methods for the Purification of Shiga Toxin 1 (pp. 187-196)
    Nutikka, Anita; Binnington-Boyd, Beth; Lingwood, Clifford A.
16. Methods for the Identification of Host Receptors for Shiga Toxin (pp. 197-208)
    Nutikka, Anita; Binnington-Boyd, Beth; Lingwood, Clifford A.
17. Shiga Toxin B-Subunit as a Tool to Study Retrograde Transport (pp. 209-220)
    Mallard, Frederic; Johannes, Ludger
18. Measuring pH Within the Golgi Complex and Endoplasmic Reticulum Using Shiga Toxin (pp. 221-228)
    Kim, Jae H.
19. Detection of Shiga Toxin-Mediated Programmed Cell Death and Delineation of Death-Signaling Pathways (pp. 229-242)
    Jones, Nicola L.
20. Interaction of Shiga Toxin with Endothelial Cells (pp. 243-262)
    Bitzan, Martin; te Loo, D. Maroeska W. M.
21. Shiga Toxin Interactions with the Intestinal Epithelium (pp. 263-274)
    Thorpe, Cheleste M.; Hurley, Bryan P.; Acheson, David W. K.
22. Protocols to Study Effects of Shiga Toxin on Mononuclear Leukocytes (pp. 275-290)
    Menge, Christian
23. Animal Models for STEC-Mediated Disease (pp. 291-306)
    Melton-Celsa, Angela R.; O???Brien, Alison D.
24. Gnotobiotic Piglets as an Animal Model for Oral Infection with O157 and Non-O157 Serotypes of STEC (pp. 307-328)
    Gunzer, Florian; Hennig-Pauka, Isabel; Waldmann, Karl-Heinz; Mengel, Michael
25. Bovine Escherichia coli O157:H7 Infection Model (pp. 329-338)
    Dean-Nystrom, Evelyn A.
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