Library Science

A cutting-edge collection of updated and core techniques for the neurological study of drugs of abuse. Described in step-by-step detail by hands-on experts, these readily reproducible protocols cover a wide variety of coherent methods for gathering information on quantitative changes in protein and mRNA at both tissue and cellular levels. There are various methods for detecting single and multiple alterations in single and multiple gene expression, for analyzing the functional roles of genes and proteins, for studying the release kinetics of striatal dopamine, and for the quantitative measurement of such neurotransmitters as acetylcholine. Insightful review articles address key current topics on the effect of drugs of abuse on the brain.

Authors: Wang, John Q.

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Table of Contents

1. The Temporal Sequence of Changes in Gene Expression by Drugs of Abuse (pp. 03-12)
   Kalivas, Peter W.; Toda, Shigenobu; Bowers, M. Scott; Baker, David A.; Ghasemzadeh, M. Behnam
2. Effects of Psychomotor Stimulants on Glutamate Receptor Expression (pp. 13-32)
   Wolf, Marina E.
3. Adult Neural Stem/Progenitor Cells in the Forebrain: Implications for Psychostimulant Dependence and Medication (pp. 33-42)
   Wang, John Q.; Mao, Limin; Lau, Yuen-Sum
4. Neuroprotective Effect of Naloxone in Infl ammation-Mediated Dopaminergic (pp. 43-54)
   Liu, Bin; Hong, Jau-Shyong
5. Neuropharmacology and Neurotoxicity of 3,4-Methylenedioxymethamphetamine (pp. 55-74)
   Gudelsky, Gary A.; Yamamoto, Bryan K.
6. Learning and Memory Mechanisms Involved in Compulsive Drug Use and Relapse (pp. 75-102)
   Berke, Joshua D.
7. From Drugs of Abuse to Parkinsonism: The MPTP Mouse Model of Parkinson???s Disease (pp. 103-116)
   Lau, Yuen-Sum; Meredith, Gloria E.
8. In Situ Hybridization with Isotopic Riboprobes for Detection of Striatal Neuropeptide mRNA Expression After Dopamine Stimulant Administration (pp. 119-136)
   Hurd, Yasmin L.
9. Combining In Situ Hybridization with Retrograde Tracing and Immunohistochemistry for Phenotypic Characterization of Individual Neurons (pp. 137-152)
   Moine, Catherine Le
10. Analysis of mRNA Expression Using Double In Situ Hybridization Labeling with Isotopic and Nonisotopic Probes (pp. 153-160)
    Wang, John Q.
11. Quantifi cation of mRNA in Neuronal Tissue by Northern Analysis (pp. 161-180)
    Konradi, Christine L.
12. Analysis of Gene Expression in Striatal Tissue by Multiprobe RNase Protection Assay (pp. 181-192)
    Richtand, Neil M.
13. Analysis of mRNA Expression in Striatal Tissue by Differential Display Polymerase Chain Reaction (pp. 193-210)
    Berke, Joshua D.
14. Semiquantitative Real-Time PCR for Analysis of mRNA Levels (pp. 211-228)
    Walker, Stephen J.; Worst, Travis J.; Vrana, Kent E.
15. Application of TaqMan RT-PCR for Real-Time Semiquantitative Analysis of Gene Expression in the Striatum (pp. 229-242)
    Medhurst, Andrew D.; Pangalos, Menelas N.
16. Systematic Screening of Gene Expression Using a cDNA Macroarray (pp. 243-260)
    Worst, Travis J.; Freeman, Willard M.; Walker, Stephen J.; Vrana, Kent E.
17. Quantification of Protein in Brain Tissue by Western Immunoblot Analysis (pp. 263-272)
    Konradi, Christine L.
18. Immunohistochemical and Immunocytochemical Detection of Phosphoproteins in Striatal Neurons (pp. 273-282)
    Mao, Limin; Wang, John Q.
19. Analysis of Protein Expression in Brain Tissue by ELISA (pp. 283-296)
    Bennett, Steffany A. L.; Roberts, David C. S.
20. Dopamine Receptor Binding and Quantitative Autoradiographic Study (pp. 297-314)
    Levant, Beth
21. Analysis of DNA-Binding Activity in Neuronal Tissue with the Electrophoretic Mobility-Shift Assay (pp. 315-328)
    Konradi, Christine L.
22. Viral-Mediated Gene Transfer to Study the Behavioral Correlates of CREB Function (pp. 331-350)
    Carlezon, William A.; Neve, Rachael L.
23. Generating Gene Knockout Mice for Studying Mechanisms Underlying Drug Addiction (pp. 351-364)
    Zhang, Jianhua; Xu, Ming
24. Antisense Approaches in Analyzing the Functional Role of Proteins in the Central Nervous System (pp. 365-376)
    Wang, John Q.; Mao, Limin
25. Primary Striatal Neuronal Culture (pp. 379-386)
    Mao, Limin; Wang, John Q.
26. Primary Rat Mesencephalic Neuron-Glia, Neuron-Enriched, Microglia-Enriched, and Astroglia-Enriched Cultures (pp. 387-396)
    Liu, Bin; Hong, Jau-Shyong
27. Primary Culture of Adult Neural Progenitors (pp. 397-404)
    Bennett, Steffanu A. L.; Melanson-Drapeau, Lysanne
28. Organotypic Culture of Developing Striatum: Pharmacological Induction of Gene Expression (pp. 405-412)
    Liu, Fu-Chin
29. Microdialysis Coupled with Electrochemical Detection: A Way to Investigate Brain Monoamine Role in Freely Moving Animals (pp. 415-432)
    Carboni, Ezio
30. HPLC/EC Detection and Quantifi cation of Acetylcholine in Dialysates (pp. 433-442)
    Zackheim, James A.; Abercrombie, Elizabeth D.
31. Real-Time Measurements of Phasic Changes in Extracellular Dopamine Concentration in Freely Moving Rats by Fast-Scan Cyclic Voltammetry (pp. 443-464)
    Phillips, Paul E. M.; Robinson, Donita L.; Stuber, Garret D.; Carelli, Regina M.; Wightman, R. Mark
32. Measurement of Dopamine Uptake in Neuronal Cells and Tissues (pp. 465-472)
    Lau, Yuen-Sum
33. Laboratory Analysis of Behavioral Effects of Drugs of Abuse in Rodents (pp. 475-480)
    Richtand, Neil M.
34. Conditioned Place Preference: A Simple Method for Investigating Reinforcing Properties in Laboratory Animals (pp. 481-498)
    Carboni, Ezio; Vacca, Cinzia
35. Detection of Cell Proliferation and Cell Fate in Adult CNS Using BrdU Double-Label Immunohistochemistry (pp. 499-506)
    Khaing, Zin Z.; Blum, Mariann

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