Library Science

Leading drosophilists describe in step-by-step detail all the essential techniques for studying Drosophila chromosomes and suggest new avenues for scientific exploration. The chapters emphasize specimen preparation (from dissection to mounting) and cover both polytene and mitotic/meiotic chromosomes in depth. Each fully tested and readily reproducible protocol offers a background introduction, equipment and reagent lists, and tips on troubleshooting and avoiding pitfalls. A cutting-edge FISH and immunolocalization technique will be important for discovering how DNA sequence influences higher-order chromosome architecture and ultimately gene expression.

Ed. Henderson, Daryl S.

 drosophila-cytogenetics-protocols.pdf

Table of Contents

1. RNAi in Cultured Drosophila Cells (pp. 1-44)
   Kao, Ling-Rong; Megraw, Timothy L.
2. Spermatogenesis: Analysis of Meiosis and Morphogenesis (pp. 45-76)
   White-Cooper, Helen
3. Time-Lapse Imaging of Male Meiosis by Phase-Contrast and Fluorescence Microscopy (pp. 77-88)
   Rebollo, Elena; Gonzalez, Cayetano
4. Immunocytological Analysis of Oogenesis (pp. 89-128)
   Mathe, Endre
5. Cytological Analysis of Oogenesis (pp. 129-138)
   Nokkala, Seppo; Nokkala, Christina
6. Polytene Chromosomes From Ovarian Nurse Cells of Drosophila melanogaster otu Mutants (pp. 139-162)
   Koryakov, Dmitry E.; Mal’ceva, Natalia I.; King, Robert C.; Zhimulev, Igor F.
7. Feulgen-DNA Cytophotometry for Estimating C Values (pp. 163-202)
   Rasch, Ellen M.
8. Fluorescent BrdU Labeling and Nuclear Flow Sorting of the Drosophila Ovary (pp. 203-214)
   Calvi, Brian R.; Lilly, Mary A.
9. Studying Nuclear Organization in Embryos Using Antibody Tools (pp. 215-234)
   Johansen, Kristen M.; Johansen, Jorgen
10. Whole-Mount Fluorescence In Situ Hybridization to Chromosomes of Embryos (pp. 235-248)
    Henderson, Daryl S.
11. Orcein Staining and the Identification of Polytene Chromosomes (pp. 249-256)
    Tonzetich, John
12. Salivary Chromosome Analysis of Aberrations (pp. 257-278)
    Carpenter, Adelaide T. C.
13. In Situ Hybridization to Polytene Chromosomes (pp. 279-288)
    Saunders, Robert D. C.
14. Combined Immunostaining and FISH Analysis of Polytene Chromosomes (pp. 289-304)
    Lavrov, Sergey; Dejardin, Jerome; Cavalli, Giacomo
15. Electron Microscopy of Polytene Chromosomes (pp. 305-324)
    Semeshin, Valery F.; Belyaeva, Elena S.; Shloma, Victor V.; Zhimulev, Igor F.
16. Analysis of Mitosis in Squash Preparations of Larval Brains: Orcein, Giemsa, Hoechst 33258, DAPI, Quinacrine, and N-Banding (pp. 325-332)
    Fanti, Laura; Pimpinelli, Sergio
17. Analyzing Chromosome Function by High Frequency Formation of Dicentric Chromosomes In Vivo (pp. 333-342)
    Ahmad, Kami; Golic, Kent G.
18. Analysis of Chromosomes of the Larval CNS by FISH and BrdU Labeling (pp. 343-352)
    Csink, Amy K.
19. Immunostaining of Squash Preparations of Chromosomes of Larval Brains (pp. 353-362)
    Fanti, Laura; Pimpinelli, Sergio
20. Visualizing Mitosis in Whole-Mount Larval Brains (pp. 363-372)
    Henderson, Daryl S.
21. Immunostaining of Whole-Mount Imaginal Discs (pp. 373-388)
    Phalle, Brigitte de Saint
22. Wing Somatic Mutation and Recombination Test (pp. 389-412)
    de Andrade, Heloisa Helena Rodrigues; Reguly, Maria Luiza; Lehmann, Mauricio
23. Analysis of Histoblasts (pp. 413-430)
    Madhavan, Mekkara Mandaravally; Madhavan, Kornath
24. Visualizing Apoptosis (pp. 431-442)
    McCall, Kimberly; Baum, Jason S.; Cullen, Kristen; Peterson, Jeanne S.
25. RNAi in Cultured Drosophila Cells (pp. 443-458)
    Kao, Ling-Rong; Megraw, Timothy L.

This entry was posted on Wednesday, April 4th, 2007 at 2:56 pm and is filed under Library. You can follow any responses to this entry through the RSS 2.0 feed. You can leave a response, or trackback from your own site.