Library Science

DNA Sequencing Protocols provides you with the knowledge to become a sequencing expert. An all-star cast of investigators covers virtually all aspects, including developments in cycle sequencing, sequencing PCR products, sequencing lambda and cosmids, multiplex sequencing, direct blotting electrophoresis, sequencing of chemiluminescence, and automated sequencing.

Authors: Griffin, Annette M.; Griffin , Hugh G.

Download Book (PDF 21863K)

Table of Contents

1. DNA Sequencing (pp. 1-8)
   Grifin, Hugh G.; Grifin, Annette M.
2. M13 Cloning Vehicles: Their Contribution to DNA Sequencing (pp. 9-22)
   Messing, Joachim
3. Cloning into Ml3 (pp. 23-30)
   Yu, Qingzhong
4. Transfection of E. coli with M13 DNA (pp. 31-36)
   Tomley, Fiona M.
5. Ml3 Phage Growth and Single-Strand DNA Preparation (pp. 37-40)
   Tomley, Fiona M.
6. M13 Phage Growth and DNA Purification Using 96 Well Microtiter Trays (pp. 41-46)
   Bankier, Alan T.
7. Generation of Random Fragments by Sonication (pp. 47-50)
   Bankier, Alan T.
8. Generation of a Nested Set of Deletions Using Exonuclease III (pp. 51-60)
   Murphy, George
9. Sequential Deletions of Single-Stranded DNA ??�Cyclone Sequencing??� (pp. 61-68)
   Murphy, George
10. Subcloning for DNA Sequencing (pp. 69-74)
    Studnicka, Gary M.; Lei, Shau-Ping; Lin, Hun-Chi; Wilcox, Gary
11. Sequencing Using Custom Designed Oligonucleotides (pp. 75-82)
    Gerischer, Ulrike; Durre, Peter
12. Dideoxy Sequencing Reactions Using Klenow Fragment DNA Polymerase 1 (pp. 83-90)
    Bankier, Alan T.
13. Dideoxy Sequencing Reactions Using T7 Polymerase (pp. 91-102)
    Durre, Peter; Geriscker, Ulrike
14. Dideoxy Sequencing Reactions Using Sequenase Version 2.0 (pp. 103-108)
    Griffin, Hugh G.; Griffin, Annette M.
15. Dideoxy Sequencing Reactions Using Taq Polymerase (pp. 109-114)
    Arigoni, Fabrizio; Kaminski, P. Alexandre
16. Pouring Linear and Buffer-Gradient Sequencing Gels (pp. 115-120)
    Littlebury, Paul
17. Electrophoresis of Sequence Reaction Samples (pp. 121-130)
    Bankier, Alan T.
18. Plasmid Sequencing (pp. 131-136)
    Griffin, Hugh G.; Griffin, Annette M.
19. Plasmid Sequencing (pp. 137-140)
    Murphy, George
20. Direct Sequencing of Inserts Cloned into Lambda Vectors (pp. 141-148)
    Malik, Afshan N.
21. Cosmid Sequencing (pp. 149-168)
    Craxton, Molly
22. Genomic Sequencing (pp. 169-182)
    Pfeifer, Gerd P.; Riggs, Arthur D.
23. Sequencing of Double-Stranded PCR Products (pp. 183-190)
    Gal, Susannah
24. Sequencing Double-Stranded Linear DNA with Sequenase and [a-35S] d.ATP (pp. 191-198)
    Casanova, Jean-Laurent
25. Solid Phase PCR Sequencing of Biotinylated Products (pp. 199-208)
    Green, Andrew; Vaudin, Mark
26. Cycle Sequencing (pp. 209-218)
    Blakesley, Robert W.
27. Direct Blotting Electrophoresis (pp. 219-224)
    Beck, Stephan
28. Multipllex DNA Sequencing (pp. 225-234)
    Beck, Stephan
29. DNA Sequencing by Chemiluminescent Detection (pp. 235-242)
    Beck, Stepkan
30. Reverse Sequencing of M13 Cloned DNA (pp. 243-246)
    Bankier, Alan T.
31. Terminal Labeling of DNA for Maxam and Gilbert Sequencing (pp. 247-254)
    Pichersky, Eran
32. DNA Sequencing by the Chemical Method (pp. 255-260)
    Pichersky, Eran
33. DNA Sequencing by Chemical Degradation Using One, Two, and Four Different Fluorophores (pp. 261-280)
    Rosenthal, Andre
34. Linear Amplification Sequencing with Dye Terminators (pp. 281-296)
    Rosenthal, Andre; Charnock-Jones, D. Stephen
35. Sequencing Reactions for the Applied Biosystems 373A Automated DNA Sequencer (pp. 297-316)
    Halloran, Nicolette; Du, Zijin; Wilson, Richard K
36. Sequencing Reactions for ALF (EMBL) Automated DNA Sequencer (pp. 317-356)
    Ansorge, Wilhelm; Zimmermann, Jurgen; Erfle, Holger; Hewitt, Neil; Rupp, Thomas; Schwager, Christian; Sproat, Brian; Stegemunn, Josef; Voss, Hartmut
37. Sequencing Using the Du Pont Genesis??? 2000 DNA Analysis System (pp. 357-372)
    Hall, Len
38. The Use of Robotic Workstations in DNA Sequencing (pp. 373-384)
    Bankier, Alan T.

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