Diagnostic Virology Protocols
posted in Library, Molecular Medicine |
A collection of cutting-edge techniques for detecting most of the major viruses that afflict mankind, including influenza, hepatitis, herpes, polio, mumps, HIV, and many more. The techniques are well-tested, easily reproducible, and readily employ all the new technologies-PCR, RIA, ELISA, and latex-agglutination-that have revolutionized the field. These methods not only make it possible to do the necessary analysis in hours instead of days, but can also be automated in a laboratory havng only low levels of biological containment. Frequently, the protocols for viruses causing human diseases can be adapted to similar viruses of veterinary importance. Through its state-of-the-art methods a physician can, for the first time, determine early in a viral infection which antiviral drug should be used and minimize the period of treatment to avoid unnecessary side effects.
Ed. Stephenson, John R. ; Warnes, Alan Table of Contents
- Rapid Detection of Adenovirus from Fecal Specimens (pp. 1-6)
Tabish, Tanvir; Warnes, Alan; Clark, Stuart - Alphaviruses (pp. 7-18)
Roehrig, John T.; Brown, Teresa M.; Johnson, Alison J.; Karabatsos, Nick; Martin, Denise A.; Mitchell, Carl J.; Nasci, Roger S. - Detection of Human Caliciviruses and Astroviruses in Stools by RT-PCR (pp. 19-28)
Jiang, Xi; Matson, David O. - Enteroviruses and Rhinoviruses (pp. 29-50)
Muir, Peter - Rapid Detection and Identification of Dengue Viruses by Reverse Transcriptase/Polymerase Chain Reaction (pp. 51-62)
Trent, Dennis W.; Chang, Gwong-Jen; Vorndam, A. Vance; Lanciotti, Robert S. - Hepatitis Viruses (pp. 63-88)
Kotwal, Girish J. - Herpesviruses (pp. 89-102)
Espy, Mark J.; Mitchell, P. Shawn; Persing, David H.; Smith, Thomas F. - Diagnosis and Direct Automated Sequencing of HIV-1 From Dried Blood Spots (DBS) Collected on Filter Paper (pp. 103-118)
Cassol, Sharon; Read, Stanley; Weniger, Bruce G.; Pilon, Richard; Leung, Barbara; Mo, Theresa - PCR for the Detection of Influenza Viruses in Clinical Material (pp. 119-128)
Ellis, Joanna S.; Brown, David W. G. - Lyssaviruses: Special Emphasis on Rabies Virus (pp. 129-142)
Bourhy, Herve - Methods for Detecting Antimeasles, Mumps, and Rubella Virus Antibodies (pp. 143-158)
Julkunen, llkka; Davidkin, lrja; Oker-Blom, Christian - Papillomaviruses (pp. 159-172)
Kirk, Sandra H.; Liu, David T. Y. - Diagnosis of Parvovirus B19-DNA by Dot-Blot Hybridization (pp. 173-188)
Hicks, Karen E.; Beard, Stuart; Cohen, Bernard J.; Clewley, Jonathan P. - Polioviruses: Concurrent Serotyping and lntratypic Differentiation of Polioviruses (pp. 189-198)
Wood, David J. - Poxviruses (pp. 199-212)
Meyer, Hermann; Ropp, Susan L.; Esposito, Joseph J. - Respiratory Syncytial Virus and Other Pediatric Respiratory Virus Infections (pp. 213-222)
Toms, G. L. - Detection and Molecular Epidemiology of Rotavirus by RNA Gel Electrophoresis (pp. 223-230)
Svensson, Lennart - Detect ion of PCR- Amplif ied Sandf ly Fever Sicilian Virus RNA by Calorimetric Enzymatic Immunoassay (pp. 231-244)
lbrahim, M. Sofi; Schmaljohn, Connie S. - Time-Resolved Fluorescence (pp. 245-256)
Halonen, Pekka; Lovgren, Timo - Enzyme-Linked lmmunosorbent Assays (pp. 257-276)
Kemeny, David M. - Dynamic Analysis of Viral Populations by Direct DNA Sequencing and Solid-Phase Technology (pp. 277-286)
Lundeberg, Joakim; Odeberg, Jacob; Uhlen, Mathias - Robotic Analyzers (pp. 287-300)
Pfost, Dale R.; Keightley, Richard G. - The Detection of Enteroviruses in Water and Associated Materials Using the Polymerase Chain Reaction (pp. 301-314)
Wyn-Jones, Peter; Sellwood, Jane - Recombinant Antigens in Viral Diagnosis (pp. 315-330)
Stephenson, John R.; Warnes, Alan - NASBA: A Method for Nucleic Acid Diagnostics (pp. 331-340)
van Strijp, Dianne; van Aarle, Pierre - Oligonucleotides (pp. 341-366)
Syrjanen, Stina