Library Science

Diagnostic Bacteriology Protocols presents a broad range of currently used techniques for detecting, identifying, and differentiating bacterial cell components, including structured proteins, nucleic acids, enzyme activities, lipopolysaccharides, and metabolites. It describes each technique in simple easy-to-follow steps that guarantee reproducible results for novices and senior researchers alike. Troubleshooting tips, alternative ways of performing procedures, and informative explanations about why certain steps are necessary-aids not usually found in standard journal recipes help even highly skilled researchers to obtain the results they want.

Authors: Howard, Jenny; Whitcombe, David M.

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Table of Contents

1. Antibiotic Resistance Profiling (pp. 1-4)
   Hammonds, Stephen J.
2. Bacteriocin Typing (pp. 5-14)
   Pitt, Tyrone L.; Gaston, Michael A.
3. Bacteriophage Typing (pp. 15-26)
   Pitt, Tyone L.; Gaston, Michael A.
4. The Analysis of Bacterial Proteins by SDS Polyacrylamide Gel Electrophoresis (pp. 27-40)
   Costas, Menelaos
5. Lipopolysaccharide Chemotyping (pp. 41-48)
   Chart, Henrik
6. Analysis of Bacterial Outer Membrane Proteins (pp. 49-62)
   Chart, Henrik
7. Multilocus Enzyme Electrophoresis (pp. 63-78)
   Boerlin, Patrick; Piffaretti, Jean-Claude
8. Fast Atom Bombardment-Mass Spectrometry (pp. 79-96)
   Drucker, David B.
9. Pyrolysis Mass Spectrometry (pp. 97-106)
   Freeman, Roger; Sisson, Penelope R.; Heatherington, Clive S.
10. The Direct Immunofluorescent Filter Technique (DIFT) (pp. 107-112)
    Kroll, Rohan
11. The Direct Epifluorescent Filter Technique (DEFT) (pp. 113-122)
    Kroll, Rohan
12. Use of Commercially Available ELISA Kits for Detection of Foodborne Pathogens (pp. 123-132)
    Robison, Barbara J.
13. Detection of Foodborne Pathogens Using DNA Probes and a Dipstick Format (pp. 133-140)
    Groody, E. Patrick
14. Preparation of Bacterial Genomic DNA (pp. 141-148)
    Bickley, Jane; Owen, Robert J.
15. Pulsed Field Gel Electrophoresis (pp. 149-164)
    Hillier, Alan J.; Davidson, Barrie E.
16. Application of Total DNA Restriction Pattern Analysis to Identification and Differentiation of Bacterial Strains (pp. 165-180)
    Giovannetti, Luciana; Ventura, Stefano
17. Determination of rRNA Gene Restriction Patterns (pp. 181-200)
    Grimont, Francine; Grimont, Patrick A. D.
18. Preparation of Nonradioactive DNA Probes (pp. 201-212)
    Eley, Adrian; Oxley, Kevin M.
19. Preparation of Bacterial Plasmid DNA (pp. 213-224)
    Tully, Michael
20. Plasmid Profile Typing and Plasmid Fingerprinting (pp. 225-236)
    Threlfall, E. John; Woodford, Neil
21. Polymerase Chain Reaction for the Detection of Listeria Species and Listeria monocytogenes (pp. 237-246)
    Siggens, Kenneth W.
22. Development of Bacterial Species-Specific DNA Probes Based on Ribosomal RNA Genes Using PCR (pp. 247-256)
    Smith, Terry J.; Maher, Majella; Gannon, Frank; Dawson, Michael T.
23. Identification of Microorganisms Using Random Primed PCR (pp. 257-268)
    Mileham, Alan J.
24. Ligase Chain Reaction (pp. 269-278)
    Shimer, George H.; Backman, Keith C.

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