Library Science

Stephen P Robinson, MD, PhD, and Andrew Stagg, PhD, have brought together a wide range of time-proven methods for studying these dendritic cells. Many of these readily reproducible techniques deal with the problem of obtaining sufficient dendritic cells for analysis, whether by isolation from a wide vareity of tissues, or from various progenitor cell populations. Other methods describe in step-by-step fashion the techniques commonly used for analyzing aspects of dendritic cells, ranging from cell migration to antigen uptake and T cell stimulation. In addition, a few techniques explore the practical challenges involved in using dendritic cells in a clinical setting to develop novel immunotherapeueutics.

Authors: Robinson, Stephen P. ; Stagg, Andrew J.

Table of Contents

1. Isolation of Dendritic Cells from Mouse Lymph Nodes (pp. 3-7)
   Gabrilovich, Dmitry
2. Isolation of Mouse Spleen Dendritic Cells (pp. 9-22)
   Stagg, Andrew J.; Burke, Fiona; Hill, Suzanne; Knight, Stella C.
3. Isolation of Mouse Thymic Dendritic Cells (pp. 23-28)
   Anjuere, Fabienne; Ardavin, Carlos
4. Isolation of Dendritic Cells from Rat Intestinal Lymph and Spleen (pp. 29-41)
   MacPherson, Gordon; Wykes, Michelle; Huang, Fang-Ping; Jenkins, Chris
5. Isolation, Enrichment, and Culture of Murine Epidermal Langerhans Cells (pp. 43-62)
   Koch, Franz; Kampgen, Eckhart; Schuler, Gerold; Romani, Nikolaus
6. Murine Intestinal Dendritic Cell Isolation (pp. 63-72)
   Pavli, Paul
7. Identification and Isolation of Rodent Lung Dendritic Cells (pp. 73-83)
   Stumbles, Philip A.; Thomas, Jennifer; Holt, Patrick G.
8. Isolation and Propagation of Mouse Liver-Derived Dendritic Cells (pp. 85-95)
   Lu, Lina; Drakes, Maureen L.; Thomson, Angus W.
9. Identification and Immunophenotypic Analyses of Peripheral Blood Dendritic Cells (pp. 99-109)
   Robinson, Stephen P.
10. Purification of Dendritic Cells from Peripheral Blood (pp. 111-120)
    Patterson, Steven; Rae, Aaron; Donaghy, Heather
11. Isolation of Human Tonsillar Dendritic Cells (pp. 121-136)
    Cameron, Paul U.; Stent, Gianna
12. Isolation of Dendritic Cells from Human Afferent Lymph (pp. 137-144)
    Brand, Christoph U.; Yawalkar, Nikhil; Hunger, Robert E.; Braathen, Lasse R.
13. Isolation of Human Skin Dendritic Cells by In Vitro Migration (pp. 145-153)
    Richters, Cornelia D.; Hoekstra, Matthias J.; Pont, John S. du; Kreis, Robert W.; Kamperdijk, Eduard W. A.
14. Human Intestinal Dendritic Cell Isolation (pp. 155-162)
    Pavli, Paul
15. Isolation of Human Lung Dendritic Cells (pp. 163-173)
    van den Heuvel, Michel M.; Havenith, Carin E. G.; Beelen, Robert H. J.
16. Identification and Isolation of Synovial Dendritic Cells (pp. 175-187)
    Pettit, Allison R.; Cavanagh, Lois; Thomas, Ranjeny
17. Generation of Murine Bone-Marrow-Derived Dendritic Cells (pp. 191-198)
    Maroof, Asher
18. Generation of Dendritic Cells from Rat Bone Marrow (pp. 199-205)
    Powell, Timothy; Major, James R.; MacPherson, Gordon
19. Derivation of Murine Dendritic Cells from Thymic Precursors (pp. 207-216)
    Lucas, Karen; Saunders, Dolores; Wu, Li
20. Generation of Mouse Dendritic Cell Lines (pp. 219-230)
    Citterio, Stefania; Rescigno, Maria; Foti, Maria; Granucci, Francesca; Matyszak, Malgosia; Girolomoni, Giampiero; Ricciardi-Castagnoli, Paola
21. Generation of Langerhans Cell-Like Dendritic Cells from Murine Fetal Skin (pp. 231-241)
    Jakob, Thilo; Udey, Mark C.
22. Derivation of Dendritic Cell Lines from Mouse Skin (pp. 243-254)
    Takashima, Akira; Bergstresser, Paul R.
23. Propagation of Human Dendritic Cells In Vitro (pp. 257-273)
    Caux, Christophe; Dubois, Bertrand
24. Generation of Dendritic Cells from Lymphoid Precursors (pp. 275-281)
    Galy, Anne
25. Dendritic Cell Generation from Highly Purified CD14+ Monocytes (pp. 283-296)
    Pickl, Winfried F.; Majdic, Otto; Knapp, Walter
26. Generation of Blood-Derived Human Dendritic Cells for Antitumor Immunotherapy (pp. 297-303)
    Gilliet, Michel F.; Nestle, Frank O.
27. Chemotaxis of In Vitro Cultured Human Dendritic Cells (pp. 307-312)
    Allavena, Paola; Mantovani, Alberto; Sozzani, Silvano; Luini, Walter; D???Amico, Giovanna; Bernasconi, Sergio
28. Adhesion of Dendritic Cells to Endothelia (pp. 313-323)
    Brown, K. Alun
29. Transendothelial Migration and Reverse Transmigration of In Vitro Cultured Human Dendritic Cells (pp. 325-330)
    Bianchi, Giancarlo; D???Amico, Giovanna; Sozzani, Silvano; Mantovani, Alberto; Allavena, Paola
30. In Vivo Assays of Langerhans Cell Migration (pp. 331-346)
    Cumberbatch, Marie; Dearman, Rebecca J.; Kimber, Ian
31. In Vivo Migration of Rat Dendritic Cells (pp. 347-368)
    Matsuno, Kenjiro; Ezaki, Taichi
32. Antigen Uptake by Dendritic Cells (pp. 369-376)
    Caux, Christophe; Dubois, Bertrand
33. Uptake of Antigen-Antibody Complexes by Human Dendritic Cells (pp. 377-386)
    Fanger, Neil A.; Guyre, Paul M.; Graziano, Robert F.
34. Immunoelectron Microscopy of Antigen Processing in Dendritic Cells (pp. 387-411)
    Kleijmeer, Monique J.; Posthuma, George; Geuze, Hans J.
35. Purification and Characterization of MHC Class II Containing Organelles in Mouse Bone-Marrow-Derived Dendritic Cells (pp. 413-422)
    Pierre, Philippe
36. Detection of Intracellular Cytokines in Dendritic Cells (pp. 423-427)
    Kelleher, Peter
37. Adenoviral-Vector-Mediated Gene Transfer to Dendritic Cells (pp. 429-439)
    Song, Wenru; Crystal, Ronald G.
38. Stimulation of Anti-HIV-1 Cytotoxic T Lymphocytes by Dendritic Cells (pp. 441-453)
    Huang, Xiaoli; Fan, Zheng; Zheng, Lian; Rinaldo, Charles R.

This entry was posted on Friday, January 5th, 2007 at 5:36 pm and is filed under Library, Molecular Medicine. You can follow any responses to this entry through the RSS 2.0 feed. You can leave a response, or trackback from your own site.