Library Science

Hands-on laboratory experts describe for the novice investigator a host of novel technologies and molecular techniques specifically designed to study cellular immunology and promote its gene therapy applications. Presented in step-by-step detail to ensure ready reproducibility, these protocols range from flow cytometric techniques to detect cytokines and growth factors in different specimens, to methods for generating and expanding dendritic cells and hematopoietic progenitors from different origins. Although the protocols require little previous experience, even highly skilled researchers will augment their skills with many novel timesaving techniques. Ed. D. Korholz and W. Kiess

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Table of Contents

1. Primary Immunodeficiencies Caused by Defects of Cytokines and Cytokine Receptors
   (pp. 03-12) Wahn, Volker
2. Intracellular Detection of T-Cell Cytokines: Differentiation of TH1 and TH2 Cells
   (pp. 15-22) Banning, Ursula; Korholz, Dieter
3. Analysis of Cytokine Profiles in Human Skin
   (pp. 23-28) Krutmann, Jean; Mang, Renz; Grether-Beck, Susanne
4. Intracytoplasmic Detection of Proinflammatory Cytokines and Chemokines in Monocytes by Flow Cytometry
   (pp. 29-40) Schultz, Christian
5. Flow-Cytometric Immune Function Methodology for Human Peripheral Blood Dendritic Cells
   pp. 41-58() Willmann, Kerstin
6. Evaluation of the Frequency of Virus-Specific CD8+ T Cells by Cytokine Flow Cytometry
   pp. 59-70() Hoffmeister, Bodo; Kiecker, Felix; Surel, Ingolf; Khatamzas, Elham; Schuster, Volker; Volk, Hans-Dieter; Kern, Florian
7. Detection of Common Cytokine and Colony Stimulating Factor Gene Polymorphisms
   (pp. 71-94) Lehrnbecher, Thomas; Chanock, Stephen J.
8. Apoptosis Induction by TRAIL
   (pp. 95-116) Eggert, Angelika; Sieverts, Hauke; Walczak, Henning
9. Angiogenic Cytokines: Quantitative and Functional Analysis
   (pp. 117-134) Eggert, Angelika; Maris, John H.
10. Experimental Strategies for Combined Suicide and Immune Cancer Gene Therapy: An Overview
    (pp. 137-152) Kramm, Christof M.; Niehues, Tim; Rainov, Nikolai G.
11. Gene Therapy with Plasmids Encoding Cytokine- or Cytokine Receptor-IgG Chimeric Proteins
    (pp. 153-170) Piccirillo, Ciriaco A.; Prudhomme, G?rald J.
12. Interferon-ß Gene Therapy for the Treatment of Arthritis
    (pp. 171-180) Chernajovsky, Yuti; Dreja, Hanna; Triantaphyllopoulos, Kostas; Gould, David
13. Gene Gun-Based In Vivo Gene Transfer: Application to DNA Vaccination
    (pp. 181-192) Sakai, Tohru; Himeno, Kunisuke
14. Purification of the Eucaryotic Heat-Shock Proteins Hsp70 and gp96
    (pp. 193-200) von Bonin, Arne; More, Solveig H.; Breloer, Minka
15. Genetic Engineering of a Recombinant Fusion Protein Possessing an Antitumor Antibody Fragment and a TNF-a Moiety
    (pp. 201-212) Xiang, Jim; Gordon, John R.
16. Genetic Engineering of Dendritic Cells by Adenovirus-Mediated TNF-a Gene Transfer
    (pp. 213-226) Xiang, Jim; Wu, Josh
17. Retroviral Transfer of T-Cell Receptor Genes into Human Peripheral Blood Lymphocytes
    (pp. 227-234) Clay, Timothy M.; Nishimura, Michael I.
18. Gene Transfer to Articular Chondrocytes with Recombinant Adenovirus
    (pp. 235-246) Palmer, Glyn D.; Gouze, Elvire; Gouze, Jean-Noel; Betz, Oliver B.; Evans, Christopher H.; Ghivizzani, Steven C.
19. Gene Therapy of X-Linked Severe Combined Immunodeficiency
    (pp. 247-260) Hacein-Bey-Abina, Salima; Saint Basile, Genevieve de; Cavazzana-Calvo, Marina
20. Gene Transfer for Generation of Tumor and Leukemia Vaccines
    (pp. 261-278) Dilloo, Dagmar; Zibert, Andree
21. Cytokine Gene Delivery into the Central Nervous System Using Intrathecally Injected Nonreplicative Viral Vectors
    (pp. 279-290) Furlan, Roberto; Pluchino, Stefano; Marconi, Peggy C.; Martino, Gianvito
22. In Vitro Regulation of Colony Stimulating Factor-Mediated Hematopoiesis in Healthy Individuals and Patients with Different Types of Myeloproliferative Disease
    (pp. 293-310) Vraetz, Thomas; Emanuel, Peter D.; Niemeyer, Charlotte M.
23. In Vitro Generation of Dendritic Cells from Cord Blood CD34+ Hematopoietic Progenitors Cells
    (pp. 311-326) Canque, Bruno; Rosenzwajg, Michele; Gluckman, Jean Claude
24. Generation of Transgenic T Cells from Human CD34+ Cord Blood Cells
    (pp. 327-340) Verhasselt, Bruno; Naessens, Evelien; Stove, Veronique
25. Ex Vivo Expansion of Umbilical Cord Blood Cells on Feeder Layers
    (pp. 341-350) Bornhauser, Martin
26. Ex Vivo Production of Cord Blood CD34+ Derived Myeloid Precursors After Serum-Free Static Culture
    (pp. 351-362) Querol, Sergio
27. Ex Vivo Expansion of Hematopoietic Stem Cells
    (pp. 363-374) Albella, Beatriz; Segovia, Jos? Carlos; Guenechea, Guillermo; Bueren, Juan Antonio
28. Large-Scale Ex Vivo Expansion of Human Megakaryocytes for Clinical Use
    (pp. 375-386) Lefebvre, Phil; Cohen, Isaac
29. Cytokine-Mediated Expansion of Human NOD-SCID-Repopulating Cells
    (pp. 387-396) Tsuji, Kohichiro; Ueda, Takahiro; Ebihara, Yasuhiro
30. Differentiation of Human Antigen-Presenting Dendritic Cells from CD34+ Hematopoietic Stem Cells In Vitro
    (pp. 399-408) Ju, Xin-Sheng; Zenke, Martin
31. Dendritic Cell Development from Mobilized Peripheral Blood CD34+ Cells
    (pp. 409-416) Lathers, Deanne M. R.; Achille, Nicholas; Young, M. Rita I.
32. In Vitro Maturation of Dendritic Cells from Blood Progenitors
    (pp. 417-426) Matera, Lina; Galetto, Alessandra
33. Generation of Human Type 1- and Type 2-Polarized Dendritic Cells from Peripheral Blood
    (pp. 427-436) Kalinski, Pawel; Vieira, Pedro; Schuitemaker, Joost H. N.; Cai, Quan; Kapsenberg, Martien
34. Preparation of Human Dendritic Cells for Tumor Vaccination
    (pp. 437-462) Shurin, Michael R.
35. Generation of Leukemic Dendritic Cells from Patients with Acute Myeloid Leukemia
    (pp. 463-472) Mohty, Mohamad; Gaugler, B?atrice; Olive, Daniel

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