Library Science

A collection of biochemical, cellular, and molecular techniques for unraveling and quantifying the events occurring between the initial contact of a cytokine at the membrane receptor and the eventual activation of gene transcription. The techniques used include the generation of transfectants, the immunohistochemical detection of cytokines in tissue sections, and optimized staining for cytoplasmic detection. Highlights include RT-PCR of small amounts of mRNA, in situ hybridization, biosensor analysis, measurement of biological activities and standardization, immunohistochemical and single-cell detection, and receptor isolation, characterization, and crystallization.

Ed. De Ley, Marc

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Table of Contents

1. Large-Scale Generation of Plasmids that Express Type I Interferon (pp. 1-6)
   Harle, Peter; Carr, Daniel J. J.
2. Identification of trans-Acting Factors by Electrophoretic Mobility Shift Assay (pp. 7-20)
   Chan, Rebecca J.; You, Min; Feng, Gen-Sheng
3. Modulation of the Interferon-y Signal by Transfection of Cells with an Antisense-RNA Expressing Vector (pp. 21-30)
   Delrue, Leen; De Ley, Marc
4. Competitive RT-PCR to Quantify Small Amounts of mRNA (pp. 31-40)
   Aust, Gabriela
5. In Situ Hybridization for Cytokines in Human Tissue Biopsies (pp. 41-46)
   Mathey, Emily; Pollard, John; Armati, Patricia
6. Purification, Identification, and Synthesis of Chemokines (pp. 47-64)
   Menten, Patricia; Wuyts, Anja; Van Damme, Jo
7. Receptor Isolation and Characterization: From Protein to Gene (pp. 65-80)
   Novick, Daniela; Rubinstein, Menachem
8. Crystallization of Cytokine-Receptor Complexes (pp. 81-92)
   Cook, William J.; Walter, Mark R.
9. Biosensor Analysis of Receptor-Ligand Interactions (pp. 93-110)
   Wu, Sheng-Jiun; Chaiken, Irwin
10. Analysis of SH2 Ligands and Identification of Sites of Interaction (pp. 111-120)
    Baca, Manuel
11. Assays for Antiviral Activity (pp. 121-134)
    Meager, Anthony
12. Assays for Cytotoxicity (pp. 135-152)
    Meager, Anthony
13. Assays for Chemotaxis (pp. 153-166)
    Wuyts, Anja; Menten, Patricia; Van Osselaer, Nancy; Van Damme, Jo
14. In Vitro and In Vivo Assays for High-Molecular-Weight Pegylated Muteins of Granulocyte Colony-Stimulating Factor (pp. 167-176)
    Eliason, James F.; Tare, Nadine; Bowen, Sharon; Inoue, Tomoaki; Horii, Ikuo
15. Development of a Mammalian Tet-On Expression Cell Line: Glucosylceramide Synthase Regulates TNF-a-Induced Apoptosis (pp. 177-192)
    Liu, Yong-Yu; Cabot, Myles C.
16. Immunohistochemical Detection of Cytokines in Human Tissue Sections (pp. 193-200)
    Hulshof, Sandra; Dukers, Danny F.; Vos, Wim; De Groot, Corline J. A.
17. Detection of Cytokine- and Chemokine-Expressing Cells at the Single Cell Level (pp. 201-218)
    Lore, Karin; Andersson, Jan
18. Detection of Cytokine Signal Transduction “Cross-Talk” in Leukocyte Activation (pp. 219-228)
    Petty, Howard R.
19. Assay System for the Effect of Prostaglandin E2 in the Determination of Polarized Cytokine Production (pp. 229-238)
    Kuroda, Etsushi; Yamashita, Uki

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