Library Science

In Crystallographic Methods and Protocols leading specialists in the application of X-ray crystallography to biological problems provide a basic and comprehensive guide to its successful use in obtaining detailed three-dimensional structures of proteins, nucleic acids, and their complexes. These deeply experienced researchers describe in clear practical terms their proven methods for preparing samples, crystallizing them, obtaining preliminary characterizations, acquiring and analyzing data, and solving and refining structures. Their step-by-step instructions give biologically trained workers immediate access to a powerful technique essential to solving many biologically relevant problems.

Authors: Jones, Christopher; Mulloy, Barbara; Sanderson, Mark R.

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Table of Contents

1. Introduction (pp. 1-22)
   Sanderson, Mark R.
2. Overexpression, Isolation, and Crystallization of Proteins (pp. 23-54)
   Skelly, Jane V.; Madden, C. Bernadette
3. Preliminary Characterization of Crystals (pp. 55-86)
   Abdel-Meguid, Sherin S.; Jeruzalmi, David; Sanderson, Mark R.
4. Modern Methods for Rapid X-Ray Diffraction Data Collection from Crystals of Macromolecules (pp. 87-126)
   Garman, Elspeth F.
5. Use of Multiple-Wavelength Anomalous Diffraction Measurements in Ab Initio Phase Determination for Macromolecular Structures (pp. 127-152)
   Murthy, H. M. Krishna
6. Structure Determination Using Isomorphous Replacement (pp. 153-172)
   Abdel-Meguid, Sherin S.
7. Molecular Replacement Using Known Structural Information (pp. 173-204)
   Tickle, Ian J.; Driessen, Huub P. C.
8. Density Modification in X-Ray Crystallography (pp. 205-226)
   Podjarny, Alberto D.; Rees, Bernard; Urzhumtsev, Alexandre G.
9. Refinement of Protein and Nucleic Acid Structures (pp. 227-244)
   Westhof, Eric; Dumas, Philippe
10. Recent Developments for Crystallographic Refinement of Macromolecules (pp. 245-266)
    Brunger, Axel T.
11. The Crystallization and Structure Analysis of Oligonucleotide Sequences (pp. 267-292)
    Neidle, Stephen
12. Crystallography in the Study of Protein-DNA Interactions (pp. 293-318)
    Brown, David G.; Freemont, Paul S.
13. Virus Crystallography (pp. 319-364)
    Fry, Elizabeth; Logan, Derek; Stuart, David
14. Crystallization and Structure Analysis of Membrane Proteins (pp. 365-388)
    Newman, Richard

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