Library Science

Steven M. Powell, MD, has assembled a hands-on collection of cutting-edge molecular methods for studying colorectal tumors. These readily reproducible techniques range from gross microdissection of specimens to specific molecular analyses. Here both the novice and experienced researcher will find productive chromosomal analyses, including comparative genomic hybridization and FISH assays, and revealing gene expression analyses, including immunohistochemical assays, Western blotting, and microarray assays. Eminently practical, with each technique presented as if the expert in the field who had perfected it were guiding the investigator, Colorectal Cancer: Methods and Protocols provides cancer investigators with a gold standard collection of all the key genetic, cellular, and molecular biological methods for analyzing colon cancer today.

Authors: Powell,Steven M.

Table of Contents

1. Microdissection of Histologic Sections: Manual and Laser Capture Microdissection Techniques (pp. 1-13)
   Moskaluk, Christopher A.
2. Isolation of a Purified Epithelial Cell Population from Human Colon (pp. 15-20)
   Roche, James K.
3. Xenografting Human Colon Cancers (pp. 21-24)
   Harper, Jeffrey C.; Adams, Reid B.; Powell, Steven M.
4. Comparative Genomic Hybridization Technique (pp. 25-33)
   El-Rifai, Wa???el; Knuutila, Sakari
5. Fluorescence In Situ Hybridization: Application in Cancer Research and Clinical Diagnostics (pp. 35-50)
   Pack, Svetlana D.; Zhuang, Zhengping
6. Microsatellite Analysis to Assess Chromosome 18q Status in Colorectal Cancer (pp. 51-58)
   Jen, Jin
7. The SURF Technique: Selective Genetic Analysis of Microscopic Tissue Heterogeneity (pp. 59-67)
   Shibata, Darryl
8. Microsatellite Instability Testing (pp. 69-79)
   Parc, Yann R.; Halling, Kevin C.
9. Immunohistochemical Analysis for hMLH1 and hMSH2 Expression in Colorectal Cancer (pp. 81-86)
   Halling, Kevin C.; Roche, Patrick C.
10. Mutation Detection in Colorectal Cancers: Direct Sequencing of DNA Mismatch Repair Genes (pp. 87-98)
    Cunningham, Julie M.; Tester, David J.; Thibodeau, Stephen N.
11. Mutation Detection in TGF-?÷ Receptors William M. Grady, Lois L. Myeroff, Hongmei He, (pp. 99-111)
    Markowitz, Sanford
12. Direct Analysis for Familial Adenomatous Polyposis Mutations (pp. 113-128)
    Powell, Steven M.
13. I1307K Mutation Detection by Allele-Specific PCR in Familial Colorectal Cancer (pp. 129-135)
    Chan, Perry S.; Gersen, Steven L.
14. Microsatellite Analysis of the Insulin-Like Growth Factor II Receptor in Colorectal Carcinomas (pp. 137-148)
    Souza, Rhonda F.; Meltzer, Stephen J.
15. Molecular Detection of Smad2/Smad4 Alterations in Colorectal Tumors (pp. 149-165)
    Thiagalingam, Sam
16. Direct Sequencing for Juvenile Polyposis Gene SMAD4/DPC4 Mutations (pp. 167-174)
    Aaltonen, Lauri A.; Roth, Stina
17. Direct Sequencing for Peutz-Jeghers Gene LKB1 ( STK11) Mutations (pp. 175-183)
    Aaltonen, Lauri A.; Avizienyte, Egle
18. Direct Sequencing for Cowden Syndrome Gene PTEN ( MMAC1) Mutations (pp. 185-199)
    Aaltonen, Lauri A.; Roth, Stina; Eng, Charis
19. Genetic Analysis Using Microarrays (pp. 195-203)
    El-Rifai, Wa???el; Knuutila, Sakari
20. Cyclooxygenase-2 (COX-2) Protein Expression byWestern Blotting (pp. 205-211)
    Kandil, Hossam M.; DuBois, Raymond N.
21. The Immunohistochemical Method (pp. 213-229)
    Cerilli, Lisa A.; Frierson, Henry F.
22. Immunohistochemical Detection and Quantitation of Cell Surface Receptors for Prostanoids (pp. 231-238)
    Cosme, Rosana; Roche, James K.
23. Adhesion Signaling Through Integrins (pp. 239-247)
    Adams, Reid B.; Rovin, Joshua D.
24. Electrophoretic Mobility Shift Assay (EMSA) (pp. 249-257)
    Smith, Michael F.; Delbary-Gossart, Sandrine
25. Transfection Assays for Transformation of Model Colonic Cell Lines (pp. 259-265)
    Worthington, Mark T.; Luo, Roger Qi
26. Assessment of Intestinal Stem Cell Survival Using the Microcolony Formation Assay (pp. 267-273)
    Tustison, Kimberly S.; Yu, Joanne; Cohn, Steven M.
27. Gene Transfer into the Colonic Mucosa (pp. 275-283)
    Coruh, Basak; Pizarro, Theresa T.

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