Library Science

An unprecedented collection of core PCR techniques useful for the study and diagnosis of human diseases. Cutting-edge and essential for today’s diagnostic laboratories, these techniques heavily utilize nonisotopic, solution phase, and in situ amplification methods. A significant number of chapters describe applications exploiting the exquisite sensitivity of PCR in the detection of rare or single cells, as in identifying fetal cells circulating in maternal blood, preimplantation embryo diagnosis, or detecting circulating cancer cells. The book demonstrates over and over again the power of PCR-its high sensitivity, specificity, and ability to rapidly discriminate sequence variations. Ed. Lo, Y. M. Dennis

Table of Contents

1. Introduction to the Polymerase Chain Reaction (pp. 3-10)
   Lo, Y. M. Dennis
2. Setting Up a PCR Laboratory (pp. 11-17)
   Lo, Y. M. Dennis
3. Amplification from Archival Materials (pp. 21-26)
   Lo, Y. M. Dennis
4. Quantitative PCR (pp. 27-38)
   Raeymaekers, Luc
5. Mutation Screening Using PCR-SSCP: Silver Staining and Isotopic Protocols (pp. 39-50)
   Saker, Philip J.
6. Multiple Fluorescence-Based PCR-SSCP Analysis with Primer-, Post-, and Internal-Labeling (pp. 51-60)
   lwahana, Hiroyuki; ltakura, Mitsuo
7. The Amplification Refractory Mutation System (pp. 61-70)
   Lo, Y. M. Dennis
8. Artificial Restriction Fragment Length Polymorphism (A-RFLP) Analysis (pp. 71-80)
   Lo, Y. M. Dennis; Horton, Virginia A.
9. Long Range PCR (pp. 81-92)
   Waggott, William
10. Generation of Labeled Probes by PCR (pp. 93-100)
    Lo, Y. M. Dennis; An, Shu F.
11. PCR for the Detection of Minority DNA Populations (pp. 101-108)
    Lo, Y. M. Dennis
12. In Situ Amplification (pp. 109-144)
    O?Leary, John
13. Sequencing of PCR Products (pp. 145-148)
    Moss, Paul; Thein, Swee Lay
14. Reverse Transcriptase-Polymerase Chain Reaction (RT-PCR) (pp. 149-158)
    Kusec, Rajko
15. PCR Amplification of Minisatellite DNA for the Detection of Mixed Chimerism After Bone Marrow Transplantation (pp. 161-170)
    Roux, Etienne
16. Diagnosis of Mitochondrial Disorders Using the PCR (pp. 171-188)
    Morten, Karl J.
17. PCR Analysis of CD44 Variants in Tumors (pp. 189-202)
    Bolodeoku, John
18. Detection of Circulating Solid Tumor Cells by Reverse Transcriptase Polymerase Chain Reaction (pp. 203-224)
    Burchill, Susan A.
19. Detection of Circulating Tumor Cells Using lmmunobead-PCR (pp. 225-232)
    Hardingham, Jennifer E.
20. Single Cell PCR: Theory, Practice, and Clinical Applications (pp. 233-264)
    Findlay, Ian
21. PCR-Based Noninvasive Prenatal Diagnosis Using Fetal Cells in Maternal Circulation (pp. 265-274)
    Lo, Y. M. Dennis
22. Noninvasive Prenatal Diagnosis Using a Single Fetal Nucleated Erythrocyte Isolated by Micromanipulation from Maternal Blood (pp. 275-286)
    Sekizawa, Akihiko; Saito, Hiroshi; Yanaihara, Takumi
23. Diagnosis of Chromosomal Aneuploidies Using Quantitative Fluorescent PCR (pp. 287-300)
    Pertl, Barbara; Adinolfi, Matteo
24. Detection of the Hepatitis C Virus by RT-PCR (pp. 301-308)
    Healey, Christopher John; Read, Steven
25. The Application of PCR to the Detection of M. tuberculosis in Sputum Samples (pp. 309-320)
    Maher, Majella; Glennon, Maura; Cormican, Martin; Smith, Terry
26. Microsatellite Analysis in Human Disease (pp. 321-340)
    Koreth, John; Bakkenist, Christopher J.; McGee, James O?D.

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