Library Science

Stephen M. Palfrey brings together for first time a collection of detailed capillary electrophoresis protocols designed exclusively for clinical applications. Written by the leading scientists who have often perfected these methods in their own laboratories, the protocols furnish new and more powerful assays for many routine serum and blood tests now regularly performed in clinical laboratories, including serum and urine proteins, hemoglobin separation, and the detection of CSF proteins, lipoproteins, myoglobin, cryoglobulins, and cathepsin D. The protocols offered for DNA analysis include double-stranded DNA analysis, the prenatal diagnosis of Down’s syndrome, Rh D/d genotyping, the identification of mutated p53 oncogene, and the detection of microstaellite instability in cancers. Many of the methods can be automated to replace more labor intensive and costly tests that are currently used in most clinical laboratories. Clinical Applications of Capillary Electrophoresis demonstrates clearly the simplicity, versatility, and power of CE over conventional methods. Ed. Palfrey, Stephen M.

Table of Contents

1. Clinical Applications of Capillary Electrophoresis (pp. 1-10)
   Jenkins, Margaret A.
2. Serum Protein Electrophoresis (pp. 11-20)
   Jenkins, Margaret A.
3. Urine Proteins (pp. 21-28)
   Jenkins, Margaret A.
4. Electrophoresis of Cerebrospinal Fluid (pp. 29-38)
   Cowdrey, Geoffrey; Firth, Maria; Firth, Gary
5. Immunosubtraction as a Means of Typing Monoclonal and Other Proteins in Serum and Urine (pp. 39-46)
   Palfrey, Stephen M.
6. Analysis and Classification of Serum Cryoglobulins (pp. 47-52)
   Shihabi, Zak K.
7. Myoglobin Analysis (pp. 53-58)
   Shihabi, Zak K.
8. Enzyme Analysis: Cathepsin D as an Example (pp. 59-64)
   Shihabi, Zak K.
9. Quantification of Human Cytomegalovirus by Competitive PCR and Capillary Electrophoresis (pp. 65-80)
   Yu, Zhongxin; Scheer, W. Douglas; Hempe, James M.
10. Laboratory Diagnosis of Structural Hemoglobinopathies and Thalassemias by Capillary Isoelectric Focusing (pp. 81-98)
    Hempe, James M.; Craver, Randall D.
11. Serum Apolipoproteins (pp. 99-108)
    Watkins, Layle K.; Cockrill, Steven L.; Macfarlane, Ronald D.
12. Gene Dosage in Capillary Electrophoresis: Prenatal Diagnosis of Down???s Syndrome and Rh D/d Genotyping (pp. 109-120)
    Righetti, Pier Giorgio; Gelfi, Cecilia; Cossu, Gian Franco
13. Rapid Analysis of Amplified Double-Stranded DNA by Capillary Electrophoresis with Laser-Induced Fluorescence Detection (pp. 121-126)
    Liu, Ming-Sun; Chen, Fu-Tai Albert
14. Identification of Mutated p53 in Cancers by Nongel-Sieving Capillary Electrophoretic SSCP Analysis (pp. 127-138)
    Oto, Michiei
15. Detection of Microsatellite Instability in Cancers by Means of Nongel-Sieving Capillary Electrophoresis (pp. 139-152)
    Oto, Michiei
16. Serum Lamotrigine Analysis (pp. 153-156)
    Shihabi, Zak K.
17. Acetonitrile Stacking: Serum Phenobarbital as an Example (pp. 157-164)
    Shihabi, Zak K.
18. Confirmation of the Presence of Drugs of Abuse in Urine (pp. 165-176)
    Palfrey, Stephen M.
19. Steroid Analysis by Micellar Electrokinetic Capillary Chromatography (pp. 177-188)
    Mohammad, Amin A.; Petersen, John R.; Bissell, Michael G.
20. Determination of Polyamines by Capillary Electrophoresis (pp. 189-198)
    Ma, Yin Fa; Yu, Qingnan; Lin, Bingcheng
21. Urinary Oxalate and Citrate (pp. 199-202)
    Holmes, Ross P.; Kennedy, Martha
22. Plasma Nitrite and Nitrate Determination (pp. 203-208)
    Ueda, Toshiko; Maekawa, Tsuyoshi; Nakamura, Kazuyuki

This entry was posted on Sunday, December 17th, 2006 at 8:48 am and is filed under Library, Molecular Medicine. You can follow any responses to this entry through the RSS 2.0 feed. You can leave a response, or trackback from your own site.