14th
February
2007
Chromosome Analysis Protocols
posted in BioLibrary, Library, Molecular Biology |Clear, step-by-step instructions provide you with all the techniques used in both classical and molecular cytogenetics. The emphasis is on the use of human material, but the techniques are designed to be readily applicable to other animal chromosomes. Chapters feature methods used in the preparation of chromosomes, in analyzing chromosomes directly at the light-microscope level, and in molecular genetics techniques.
Authors: Gosden, John R.
Table of Contents
- Mitotic Metaphase Chromosome Preparation from Peripheral Blood for High Resolution (pp. 1-10)
Spowart, George - Chromosome Preparation from Hematological Malignancies (pp. 11-26)
Ross, Fiona M. - Meiotic Chromosome Preparation (pp. 27-40)
Chundley, Ann C.; Speed, Robert M.; Ma, Kun - Preparation of Chromosomes for Scanning Electron Microscopy (pp. 41-50)
Sumner, Adrian T.; Ross, Andrew R.; Graham, Elizabeth - Immortalized Cell Lines: Chromosome Preparation and Banding (pp. 51-58)
Fletcher, Judy - Chromosome Banding and Identification Absorption Staining (pp. 59-82)
Sumner, Adrian T. - Chromosome Banding and Identification: Fluorescence (pp. 83-96)
Sumner, Adrian T. - Chromosome Banding: Stain Combinations for Specific Regions (pp. 97-112)
Schweizer, Dieter; Ambros, Peter E - Inhibition of Chromosome Condensation (pp. 113-122)
Sumner, Adrian T.; Mitchell, Arthur R - Analysis of Chromosomes with Restriction Endonucleases and DNase Hypersensitivity (pp. 123-140)
de la Torre, Joaquina; Sumner, Adrian T. - Automatic Karyotype Analysis (pp. 141-186)
Graham, Jim; Piper, Jim - Bivariate Chromosome Analysis Using a Commercial Flow Cytometer (pp. 187-204)
Carter, Nigel P. - Chromosome Sorting by Flow Cytometry: Production of DNA Libraries and Gene Mapping (pp. 205-220)
Fantes, Judith G; Green, Daryll K.; Sharkey, Andrew - Electrophoretic Karyotype Analysis: Pulsed Field Gel Electrophoresis (pp. 221-252)
Maule, John - Immunofluorescence Techniques Applied to Mitotic Chromosome Preparations (pp. 253-286)
Jeppesen, Peter - Immunocytochemical Techniques Applied to Meiotic Chromosomes (pp. 287-302)
Heyting, Christa; Dietrich, Axel J. J.; de Jong, J. Hans; Hartsuiker, Edgar - Immortalized Cell Lines: Their Creation and Use in Gene Mapping (pp. 303-322)
van Heyningen, Veronica - Oligonucleotide Primed in Situ DNA Synthesis (PRINS): An Alternative to in Situ Hybridization for Gene Mapping and the Investigation of Genome Organization (pp. 323-334)
Gosden, John R.; Lawson, Diane - Electron Microscopic Localization of in Situ Hybrids (pp. 335-352)
Narayanswami, Sandya; Hamkalo, Barbara A. - Protocols for Chromosome-Mediated Gene Transfer: Selection Strategies, Transgenome Analysis, Enrichment Cloning, and Mapping (pp. 353-378)
Porteous, David J. - Construction of Chromosome-Specific Libraries of Yeast Artificial Chromosome Recombinants from Somatic Hybrid Cell Lines (pp. 379-402)
Arveiler, Benoit - Yeast Artificial Chromosome Recombinants in a Global Strategy for Chromosome Mapping: Amplification of Internal and Terminal Fragments by PCR, and Generation of Fingerprints (pp. 403-424)
Arveiler, Benoit - Chromosome Dissection and Cloning (pp. 425-436)
Brown, Steven D. M.; Carey, Alyson H. - Molecular Analysis of Chromosome Aberrations in Hematological Malignancies: Southern Hybridization (pp. 437-448)
Saltman, David L.; Hunger, Stephen P.; Turner, Gillian E. - Molecular Analysis of Chromosome Aberrations In Situ Hybridization (pp. 449-478)
Lichter, Peter; Ried, Thomas - Ah- and Ll-Primed PCR-Generated Probes for Nonisotopic in Situ Hybridization (pp. 479-492)
Gosden, John; Breen, Matthew; Lawson, Diane - Chromosome Substructure Investigation: Telomeres (pp. 493-504)
Allshire, Robin C.; Cooke, Howard J.