Library Science

A panel of leading scientific experts detail novel techniques and strategies for the cellular and genetic modification of heart function. The highly experienced authors provide step-by-step protocols for vector production and purification, for gene and cell delivery techniques, and for physiological assessment in vivo and in vitro. Timely, authoritative, and state-of-the-art, Cardiac Cell and Gene Transfer: Principles, Protocols, and Applications constitutes an invaluable guide to all the new cellular and gene-based technologies needed by basic and clinical investigators working to illuminate today’s unanswered questions about heart disease and ultimately to improve the heart performance in all their patients.

Ed. Metzger, Joseph M.

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Table of Contents

1. Adenoviral Vectors: Production and Purification (pp. 03-18)
   Albayya, Faris P.; Metzger, Joseph M.
2. Gutted Adenoviral Vectors for Gene Transfer to Muscle (pp. 19-28)
   Scott, Jeannine M.; Chamberlain, Jeffrey S.
3. Dual Vector Expansion of the Recombinant AAV Packaging Capacity (pp. 29-52)
   Duan, Dongsheng; Yue, Yongping; Engelhardt, John F.
4. Lentivirus Vector-Mediated Gene Transfer to Cardiomyocytes (pp. 53-70)
   Sakoda, Tsuyoshi; Kasahara, Noriyuki; Kedes, Larry
5. Cell Therapy in the Heart: Cell Production, Transplantation, and Applications (pp. 73-81)
   Cahill, Kevin S.; Toma, Catalin; Pittenger, Mark F.; Kessler, Paul D.; Byrne, Barry J.
6. Cardiac Cell Transplantation: Protocols and Applications (pp. 83-96)
   White, Steven M.; Claycomb, William C.
7. Cell Grafting for Cardiac Repair (pp. 97-112)
   Reinecke, Hans; Murry, Charles E.
8. Suppressor tRNAs: Protocols and Applications for Cardiac Gene Transfer (pp. 115-128)
   Buvoli, Massimo; Buvoli, Ada; Leinwand, Leslie A.
9. Antisense Oligonucleotides: Design, Construction, and Applications to Cardiac Allograft Transfer (pp. 129-134)
   Balsam, Leora B.; Miniati, Douglas N.; Robbins, Robert C.
10. Theoretical and Technical Considerations for Gene Transfer into Vascularized Cardiac Transplants (pp. 135-144)
    Lu, Guanyi; Bishop, D. Keith
11. Isolation, Culture, and Gene Transfer of Adult Canine Cardiac Myocytes (pp. 145-158)
    Hirsch, Jennifer C.; Borton, Andrea R.; Metzger, Joseph M.
12. Myofilament Protein Phosphorylation by PKC in Genetically Engineered Adult Cardiac Myocytes (pp. 159-166)
    Westfall, Margaret V.
13. Embryonic and Neonatal Cardiac Gene Transfer In Vivo (pp. 169-178)
    Christensen, Geir; Gruber, Peter J.; Wang, Vibin; Chien, Kenneth R.
14. Efficient Viral Gene Transfer to Rodent Hearts In Vivo (pp. 179-194)
    Monte, Federica del; Hajjar, Roger J.
15. Direct Gene Transfer to the Adult Rodent Myocardium In Vivo (pp. 195-202)
    Szatkowski, Michael L.; Westfall, Margaret V.; Metzger, Joseph M.
16. Adenovirus-Mediated Gene Transfer to Cardiac Myocytes In Vivo Using Catheter-Based Procedures (pp. 203-212)
    Logeart, Damien; Mercadier, Jean-Jacques
17. Coronary Perfusion Cocktails for In Vivo Gene Transfer (pp. 213-218)
    Lehnart, Stephan E.; Donahue, J. Kevin
18. Modification of In Vivo Cardiac Performance by Intracoronary Gene Transfer of ß-Adrenergic Receptor Signaling Components (pp. 219-232)
    Tevaearai, Hendrik T.; Koch, Walter J.
19. Protocols for Hemodynamic Assessment of Transgenic Mice In Vivo (pp. 233-243)
    Georgakopoulos, Dimitrios; Kass, David A.

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