Library Science

A collection of cutting-edge techniques for using capillary electrophoresis (CE) to analyze complex carbohydrates. These readily reproducible protocols provide methods for sample preparation, analysis of mono- and oligosaccharides, glycoproteins, and glycoconjugates. A useful appendix describes the structures of the most commonly encountered carbohydrate residues and olgosaccharides from mammalian and bacterial origins. Each protocol contains detailed information on reagents, apparatus, notes, comments, and tips on procedures.

capillary-electrophoresis-of-carbohydrates.pdf

Table of Contents

• Structural and Functional Diversity of Glycoconjugates: A Formidable Challenge to the Glycoanalyst
  (pp. 3-24)Hart, Gerald W.
• Chemical and Enzymatic Release of Glycans from Glycoproteins
  (pp. 27-40)Merry, Tony; Astrautsova, Sviatlana
• Derivatization of Carbohydrates
  (pp. 41-69)Suzuki, Shigeo; Kelly, John F.; Locke, Steven J.; Thibault, Pierre; Honda, Susumu
• Preparation of Oligosaccharides from Sulfated Glycosaminoglycans Using Bacterial Enzymes
  (pp. 71-78)Yamada, Shuhei; Sugahara, Kazuyuki
• Monosaccharide Analysis by Capillary Electrophoresis
  (pp. 81-92)Honda, Susumu
• Separation of Oligosaccharides by Capillary Electrophoresis Using Buffer Modifiers
  (pp. 93-104)Honda, Susumu; Suzuki, Shigeo
• Characterization of Oligosaccharides from Starch, Dextran, Cellulose, and Glycoproteins by Capillary Electrophoresis
  (pp. 105-120)Chen, Fu-Tai A.
• Affinity Capillary Electrophoresis of Oligosaccharides
  (pp. 121-130)Taga, Atsushi; Honda, Susumu
• Characterization of Glycosaminoglycans by Capillary Electrophoresis
  (pp. 131-144)Linhardt, Robert J.; Toida, Toshihiko
• Analysis of 2-Aminoacridone-Derivatized Complex Oligosaccharides Using Micellar Electrokinetic Capillary Chromatography and Laser-Induced Fluorescence Detection
  (pp. 147-162)Birrell, Helen C.; Camilleri, Patrick
• Separation of Protein Glycoforms by Capillary Electrophoresis
  (pp. 163-196)Taverna, Myriam; Tran, Nguyet Thuy; Ferrier, Danielle
• Separation of Glycoproteins by Capillary Isoelectric Focusing
  (pp. 197-218)Krull, Ira S.; Kazmi, Sarah; Zhong, Huijuan; Santora, Ling Chen
• Glycoprotein Analysis by Capillary Zone Electrophoresis-Electrospray Mass Spectrometry
  (pp. 219-239)Bateman, Kevin P.; Kelly, John F.; Thibault, Pierre; Ramaley, Louis; White, Robert L.
• Analysis of Bacterial Glycolipids by Capillary Electrophoresis-Electrospray Mass Spectrometry: Haemophilus influenzae and Neisseria meningitidis Lipopolysaccharides
  (pp. 241-260)Thibault, Pierre; Martin, Adele; Gilbert, Michel; Wakarchuk, Warren W.; Richards, James C.
• Capillary Electrophoresis as an Assay Method for Monitoring Glycosyltransferase Activity
  (pp. 263-274)Wakarchuk, Warren W.; Cunningham, Anna-Maria
• Determination of Association Constant of Carbohydrate-Protein Interaction
  (pp. 275-284)Taga, Atsushi; Honda, Susumu
• Appendix

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