Library Science

A collection of readily reproducible experimental methods for studying the subversion of normal cell signaling that leads to cancer. Presented in step-by-step detail to ensure successful results, the methods include proven techniques for the investigation of apoptosis and cell death, complementary protocols for manipulating and/or monitoring oncogenic signals in cancer cells, and techniques for studying protein–protein interactions. A detailed protocol for capturing pure samples of malignant cells from frozen tissue specimens and two alternative techniques for analyzing their genomic DNA is also described.

  Ed. D.M. Terrian  Download Book 

Table of Contents

1. Functional Analysis of the Antimitogenic Activity of Tumor Suppressors
   (pp. 03-16) Knudsen, Erik S.; Angus, Steven P.
2. Rescue and Isolation of Rb-deficient Prostate Epithelium by Tissue Recombination
   (pp. 17-34) Hayward, Simon W.; Wang, Yuzhuo; Day, Mark L.
3. Signal Transduction Study Using Gene-Targeted Embryonic Stem Cells
   (pp. 35-46) Kawasome, Hideki; Hamazaki, Takashi; Minamino, Tetsuo; Terada, Naohiro
4. The Use of the Yeast Two-Hybrid System to Measure Protein-Protein Interactions that Occur Following Oxidative Stress
   (pp. 47-58) Franklin, Richard A.
5. Differential Screening of cDNA Libraries for Analysis of Gene Expression During Tumor Progression
   (pp. 59-70) Gregory, Christopher W.
6. Mitogen-Activated Protein Kinase Signaling in Drug-Resistant Neuroblastoma Cells
   (pp. 71-84) Mattingly, Raymond R.
7. TUNEL and Immunofluorescence Double-Labeling Assay for Apoptotic Cells with Specific Antigen(s)
   (pp. 85-96) Oberhaus, Stephanie M.
8. Kinetworks^TM Protein Kinase Multiblot Analysis
   (pp. 99-112) Pelech, Steven; Sutter, Catherine; Zhang, Hong
9. Protein Tyrosine Kinase and Phosphatase Expression Profiling in Human Cancers
   (pp. 113-126) Lin, Wen-Chang
10. Association of Nonreceptor Tyrosine Kinase c-Yes with Tight Junction Protein Occludin by Coimmunoprecipitation Assay
    (pp. 127-132) Chen, Yan-Hua; Lu, Gun
11. Isolation of Novel Substrates Using a Tyrosine Kinase Overlay/ In Situ Assay
    (pp. 133-142) Gelman, Irwin H.
12. Manipulating Expression of Endogenous Oncogenic Proteins Using an Antisense Oligonucleotide Approach in Prostate Cancer Cells
    (pp. 143-154) Wu, Daqing; Wescott, Ginger G.; Terrian, David M.
13. Measurements of Phospholipases A2, C, and D (PLA2, PLC, and PLD): In Vitro Microassays, Analysis of Enzyme Isoforms, and Intact-Cell Assays
    (pp. 155-176) Gomez-Cambronero, Julian; Horwitz, Joel; Shaafi, Ramadan I.
14. Detection of the Content and Activity of the Transcription Factor AP-1 in a Multistage Skin Carcinogenesis Model
    (pp. 177-184) Zhao, Yunfeng; St. Clair, Daret K.
15. Fibroblastic, Hematopoietic, and Hormone Responsive Epithelial Cell Lines and Culture Conditions for Elucidation of Signal Transduction and Drug Resistance Pathways by Gene Transfer
    (pp. 185-202) Steelman, Linda S.; Blalock, William L.; Wang, Xiao-Yang; Moye, Phillip W.; Lee, John T.; Shelton, John G.; Navolanic, Patrick M.; Davis, Julianne M.; Knapp, Steven L.; Franklin, Richard A.; White, Martyn K.; McCubrey, James A.
16. Elucidation of Signal Transduction Pathways by Transfection of Cells with Modified Oncogenes
    (pp. 203-220) Steelman, Linda S.; Blalock, William L.; Wang, Xiao-Yang; Moye, Phillip W.; Lee, John T.; Shelton, John G.; Navolanic, Patrick M.; Davis, Julianne M.; Knapp, Steven L.; Franklin, Richard A.; White, Martyn K.; McCubrey, James A.
17. Elucidation of Signal Transduction Pathways by Retroviral Infection of Cells with Modified Oncogenes
    (pp. 221-252) Steelman, Linda S.; Blalock, William L.; Wang, Xiao-Yang; Moye, Phillip W.; Lee, John T.; Shelton, John G.; Navolanic, Patrick M.; Davis, Julianne M.; Knapp, Steven L.; Franklin, Richard A.; White, Martyn K.; McCubrey, James A.
18. Methods for the Study of Protein-Protein Interactions in Cancer Cell Biology
    (pp. 255-268) Price, Daniel; Park, Iha; Avraham, Hava
19. Production of Ligand-Specific Mutants Using a Yeast Two-Hybrid Mating Assay
    (pp. 269-276) Nantel, Andre
20. Coimmunoprecipitation Assay for the Detection of Kinase-Substrate Interactions
    (pp. 277-284) Comai, Lucio
21. Mutational Analysis of the Androgen Receptor Using Laser Capture Microdissection and Direct Sequencing
    (pp. 287-302) Greene, Sheila; Stockton, Patricia; Kozyreva, Olga G.; Gaston, Kris E.; Flake, Gordon P.; Mohler, James L.
22. Clonality Analysis by T-Cell Receptor PCR and High-Resolution Electrophoresis in the Diagnosis of Cutaneous T-Cell Lymphoma (CTCL)
    (pp. 303-320) Lukowsky, Ansgar

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