Library Science

Expert researchers describe in detail their most productive and up-to-date methods for growing cancer cells in the laboratory. There are methods to characterize and authenticate cell lines, to isolate and develop specific types of cancer cells, and to develop new cell line models. Functional assays are provided to evaluate clonogenicity, cell proliferation, apoptosis, adhesion, migration, invasion, senescence, angiogenesis, and cell cycle parameters. Other methods permit the modification of cancer cells for transfection, development of drug resistance, immortalization, and transfer in vivo; the co-culture of different cell types; and the detection and treatment of contamination.

Authors: Langdon, Simon P.

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Table of Contents

1. Basic Principles of Cancer Cell Culture (pp. 3-16)
   Langdon, Simon P.
2. Essential Techniques of Cancer Cell Culture (pp. 17-30)
   Macleod, Kenneth G.; Langdon, Simon P.
3. Characterization and Authentication of Cancer Cell Lines: An Overview (pp. 33-42)
   Langdon, Simon P.
4. Authentication of Cancer Cell Lines by DNA Fingerprinting (pp. 43-56)
   Dirks, Wilhelm G.; Drexler, Hans G.
5. Cytogenetic Characterization of Tumor Cell Lines (pp. 57-76)
   MacLeod, Roderick A. F.; Drexler, Hans G.
6. Isolation and Culture of Colon Cancer Cell Lines (pp. 79-92)
   Park, Jae-Gahb; Ku, Ja-Lok; Park, So-Yeon
7. Isolation and Culture of Melanoma Cell Lines (pp. 93-100)
   Riker, Adam I.
8. Isolation and Culture of Human Brain Tumor Cells (pp. 101-110)
   Gomez, German G.; Kruse, Carol A.
9. Isolation and Culture of Renal Cancer Cell Lines (pp. 111-120)
   Park, Jae-Gahb; Ku, Ja-Lok; Park, So-Yeon
10. Isolation and Culture of Prostate Cancer Cell Lines (pp. 121-132)
    Navone, Nora M.; Olive, Matilde; Troncoso, Patricia
11. Isolation and Culture of Ovarian Cancer Cell Lines (pp. 133-140)
    Langdon, Simon P.
12. Isolation and Culture of Leukemia Cell Lines (pp. 141-156)
    Drexler, Hans G.
13. Cell Sensitivity Assays: Clonogenic Assay (pp. 159-164)
    Plumb, Jane A.
14. Cell Sensitivity Assays: The MTT Assay (pp. 165-170)
    Plumb, Jane A.
15. PARP Cleavage as a Means of Assessing Apoptosis (pp. 171-182)
    Mullen, Peter
16. Detection of Apoptosis by the TUNEL Assay (pp. 183-190)
    Lawry, John
17. Apoptosis Measurement by Annexin V Staining (pp. 191-202)
    Miller, Eric
18. Cell Adhesion Assays (pp. 203-218)
    Scott, William N.
19. Radial Monolayer Cell Migration Assay (pp. 219-224)
    Berens, Michael E.; Beaudry, Christian
20. Invasion and Motility Assays (pp. 225-230)
    Scott, William N.
21. Identification of Senescence in Cancer Cells (pp. 231-238)
    Jardine, Lesley J.
22. Angiogenesis Assays (pp. 239-246)
    Scott, William N.
23. Flow Cytometric DNA Analysis of Human Cancer Cell Lines (pp. 247-256)
    Mullen, Peter
24. DNA-Mediated Gene Transfer (pp. 259-266)
    O???Donnell, Amanda J. M.
25. Development of Drug-Resistant Models (pp. 267-274)
    Coley, Helen M.
26. Immortalization of Human Prostate Cells With the Human Papillomavirus Type 16 E6 Gene (pp. 275-286)
    Maitland, Norman J.; Macintosh, Catherine A.; Schmitz, Christian; Lang, Shona H.
27. The Use of Matrigel to Facilitate the Establishment of Human Cancer Cell Lines as Xenografts (pp. 287-292)
    Mullen, Peter
28. Coculture of Prostate Cancer Cells (pp. 295-302)
    Lang, Shona H.; Maitland, Norman J.
29. Coculture of Ovarian Cells Using Porous Tissue Culture Inserts (pp. 303-306)
    Snow, Katherine E.; Langdon, Simon P.
30. Cell Culture Contamination: An Overview (pp. 309-318)
    Langdon, Simon P.
31. Detecting Mycoplasma Contamination in Cell Cultures by Polymerase Chain Reaction (pp. 319-326)
    Uphoff, Cord C.; Drexler, Hans G.
32. Elimination of Mycoplasma from Infected Cell Lines Using Antibiotics (pp. 327-334)
    Uphoff, Cord C.; Drexler, Hans G.

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