17th
January
2007
Bone Research Protocols
posted in Library |
A collection of the latest laboratory techniques for the study of bone and bone tissue. Described in step-by-step detail, these readily reproducible methods cover such topics as the isolation and culture of bone cells, the preparation of bone tissue for histological and ultrastructural analysis, methods for the measurement of bone strength and for mechanical studies, and how to use digital imaging techniques in the analysis of bone.
Authors: Helfrich, Miep H. ; Ralston, Stuart H.
Table of Contents
- Human Osteoblast Culture (pp. 3-18)
Gallagher, James A. - Osteoblast Isolation from Murine Calvariae and Long Bones (pp. 19-28)
Bakker, Astrid; Klein-Nulend, Jenneke - Mineralizing Fibroblast-Colony-Forming Assays (pp. 29-40)
Scutt, Andrew; Reading, Lynsey; Scutt, Nanette; Still, Karen - Osteocyte Isolation and Culture (pp. 41-50)
Nijweide, Peter J.; van der Plas, Arie; Alblas, Marcel J.; Klein-Nulend, Jenneke - Isolated Osteoclast Cultures (pp. 53-64)
Hoebertz, Astrid; Arnett, Timothy R. - Primary Isolation and Culture of Chicken Osteoclasts (pp. 65-88)
Collin-Osdoby, Patricia; Anderson, Fred; Osdoby, Philip - Isolation and Purification of Rabbit Osteoclasts (pp. 89-100)
Coxon, Fraser P.; Frith, Julie C.; Benford, Helena L.; Rogers, Michael J. - Generating Human Osteoclasts from Peripheral Blood (pp. 101-112)
Sabokbar, Afsie; Athanasou, Nicholas S. - Generating Human Osteoclasts In Vitro from Bone Marrow and Peripheral Blood (pp. 113-128)
Flanagan, Adrienne M.; Massey, Helen M. - Generating Murine Osteoclasts from Bone Marrow (pp. 129-144)
Takahashi, Naoyuki; Udagawa, Nobuyuki; Tanaka, Sakae; Suda, Tatsuo - Osteoclast Formation in the Mouse Coculture Assay (pp. 145-152)
van t??? Hof, Robert J. - RANKL-Mediated Osteoclast Formation from Murine RAW 264.7 Cells (pp. 153-166)
Collin-Osdoby, Patricia; Yu, Xuefeng; Zheng, Hong; Osdoby, Philip - Analysis of Osteoclast Function in Mouse Calvarial Cultures (pp. 169-182)
Marshall, Michael J.; Rowlands, Marit N. - Assessing Bone Formation Using Mouse Calvarial Organ Cultures (pp. 183-198)
Garrett, I. Ross - In Situ Hybridization and In Situ Reverse Transcription Polymerase Chain Reaction in Human Bone Sections (pp. 201-224)
Mee, Andrew P.; Hoyland, Judith A. - Techniques for the Study of Apoptosis in Bone (pp. 225-236)
Noble, Brendon S.; Stevens, Hazel Y. - Protein Localization in Wax-Embedded and Frozen Sections of Bone Using Immunohistochemistry (pp. 237-248)
Bord, Sharyn - Detection of Noncollagenous Bone Proteins in Methylmethacrylate-Embedded Human Bone Sections (pp. 249-258)
van Leeuwen, Johannes P. T. M.; Derkx, Pieter - Fluorescence Imaging of Bone-Resorbing Osteoclasts by Confocal Microscopy (pp. 259-282)
Nesbitt, Stephen A.; Horton, Michael A. - Bone Histomorphometry (pp. 283-298)
Vedi, Shobna; Compston, Juliet - Transmission Electron Microscopy of Bone (pp. 299-310)
Everts, Vincent; Niehof, Anneke; Beertsen, Wouter - Scanning Electron Microscopy of Bone (pp. 311-320)
Marshall, Deborah; Helfrich, Miep H.; Aspden, Richard M. - Bone Measurements by Peripheral Quantitative Computed Tomography in Rodents (pp. 323-342)
Gasser, Jurg A. - Studies of Local Bone Remodeling: The Calvarial Injection Assay (pp. 345-352)
van ???t Hof, Robert J.; Clarkin, Claire E.; Armour, Kenneth J. - Inflammation-Induced Osteoporosis: The IMO Model (pp. 353-360)
Armour, Kenneth J.; Armour, Katharine E. - Ovariectomy and Estrogen Replacement in Rodents (pp. 361-366)
Chow, Jade W. M. - Mechanical Testing of Bone Ex Vivo (pp. 369-380)
Aspden, Richard M. - Bone Cell Responses to Fluid Flow (pp. 381-398)
Stevens, Hazel Y.; Frangos, John A. - Methods for Analyzing Bone Cell Responses to Mechanical Loading Using In Vitro Monolayer and Organ Culture Models (pp. 399-422)
Pitsillides, Andrew A.; Das-Gupta, Victoria; Simon, Dominic; Rawlinson, Simon C. F. - Extraction of Nucleic Acids from Bone (pp. 425-432)
Stewart, Tracy L.; Mann, Val - Analysis of Gene Expression in Bone by Quantitative RT-PCR (pp. 433-440)
Ireland, Deborah