Library Science

Expert laboratory researchers describe in a standard format all the diverse laboratory methods needed to perform state-of-the-art tissue engineering. Topics range from the synthesis, processing, and characterization of specific biomaterials, through the successful use of scaffolds in the engineering of tissues, to techniques useful in evaluating the biological quality of scaffold-engineered tissues. Topics of special interest include the incorporation of biological molecules into scaffold biomaterials and the use of a wide range of methods and techniques to generate a comprehensive description of cell–polymer construct quality.

Authors: Hollander, Anthony P.; Hatton, Paul V.

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Contents

• Processing of Resorbable Poly-a-Hydroxy Acids for Use as Tissue-Engineering Scaffolds (pp. 1-10)
  Kellomaki, Minna; Tormala, Pertti
• Fibrin Microbeads (FMB) As Biodegradable Carriers for Culturing Cells and for Accelerating Wound Healing (pp. 11-24)
  Gorodetsky, Raphael; Vexler, Akiva; Levdansky, Lilia; Marx, Gerard
• Synthesis and Characterization of Hyaluronan-Based Polymers for Tissue Engineering (pp. 25-40)
  Soranzo, Carlo; Renier, Davide; Pavesio, Alessandra
• Synthesis and Characterization of Chitosan Scaffolds for Cartilage-Tissue Engineering (pp. 41-48)
  Elder, Steven H.; Nettles, Dana L.; Bumgardner, Joel D.
• Characterization of a Calcium Phosphate-Based Matrix for rhBMP-2 (pp. 49-64)
  Kim, Hyun D.; Wozney, John M.; Li, Rebecca H.
• Methodologies for Processing Biodegradable and Natural Origin Scaffolds for Bone and Cartilage Tissue-Engineering Applications (pp. 65-76)
  Gomes, Manuela E.; Malafaya, Patricia B.; Reis, Rui L.
• Alginates in Tissue Engineering (pp. 77-86)
  Wong, Marcy
• Production and Surface Modification of Polylactide-Based Polymeric Scaffolds for Soft-Tissue Engineering (pp. 87-112)
  Cao, Yang; Croll, Tristan I.; Cooper-White, Justin J.; O’Connor, Andrea J.; Stevens, Geoffrey W.
• Modification of Materials With Bioactive Peptides (pp. 113-122)
  West, Jennifer L.
• Isolation and Osteogenic Differentiation of Bone-Marrow Progenitor Cells for Application in Tissue Engineering (pp. 123-130)
  Salgado, Antonio J.; Gomes, Manuela E.; Coutinho, Olga P.; Reis, Rui L.
• Cell Seeding of Polymer Scaffolds (pp. 131-146)
  Vunjak-Novakovic, Gordana; Radisic, Milica
• Chondrocyte Isolation, Expansion, and Culture on Polymer Scaffolds (pp. 147-158)
  Crawford, Aileen; Dickinson, Sally C.
• Bioreactor Culture Techniques for Cartilage-Tissue Engineering (pp. 159-170)
  Lee, David A.; Martin, Ivan
• Microscopic Methods for the Analysis of Engineered Tissues (pp. 171-196)
  Roberts, Sally; Menage, Janis
• Transmission Electron Microscopy of Tissue-Polymer Constructs (pp. 197-206)
  Hatton, Paul V.
• Application of Microscopic Methods for the Detection of Cell Attachment to Polymers (pp. 207-216)
  Hunt, John; Heggarty, Deborah
• Biochemical Methods for the Analysis of Tissue-Engineered Cartilage (pp. 217-230)
  Kafienah, Wa’el; Sims, Trevor J.
• Real-Time Quantitative RT-PCR Assays (pp. 231-238)
  Martin, Ivan; Frank, Oliver
• Mechanical Testing of Cell-Material Constructs: A Review (pp. 239-254)
  Kisiday, John; Kerin, Alex; Grodzinsky, Alan

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