Library Science

This second volume of protocols offers the most comprehensive set of modern analytical techniques available for studying the architecture and function of membranes. It features the application of biochemical, spectroscopic, and fluorimetric methods to the investigation of molecular topology, the dynamic aspects of membrane structure, and ion transport. Antibody technology, analysis of molecules involved in intracellular signaling, and receptor???ligand interactions are also covered.

Authors: Graham, John M.; Higgins, Joan A.

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Table of Contents

1. Crystallization of Membrane Proteins for X-Ray Analysis (pp. 1-18)
   Sutton, Brian J.; Sohi, Maninder K
2. Determination of Cell-Surface Polarity by Solid-Phase Lactoperoxidase Iodination (pp. 19-30)
   Muller, William A.
3. Biochemical Methods to Determine Cell-Surface Topography (pp. 31-42)
   Muller, William A.
4. Use of Antipeptide Antibodies for the Isolation and Study of Membrane Proteins (pp. 43-64)
   Baldwin, Stephen A.
5. The Production of Monoclonal Antibodies to Membrane Proteins (pp. 65-86)
   Partridge, Lynda J.
6. Purification of a Membrane Protein (Ca2+/Mg2+-ATPase) and Its Reconstitution into Lipid Vesicles (pp. 87-94)
   East, J. Malcolm
7. Measurement of Protein-Protein Interactions in Reconstituted Membrane Vesicles Using Fluorescence Spectroscopy (pp. 95-100)
   Lee, Anthony G.
8. Measurement of Lipid-Protein Interactions in Reconstituted Membrane Vesicles Using Fluorescence Spectroscopy (pp. 101-108)
   Lee, Anthony G.
9. Determination of the Transverse Topography of Membrane Lipids Using Enzymes and Covalent Labels as Probes (pp. 109-124)
   Higgins, Joan A.
10. Determination of the Transverse Topography of Membrane Phospholipids Using Phospholipid Transfer Proteins as Tools (pp. 125-130)
    Higgins, Joan A.
11. Prothrombinase Complex as a Tool to Assess Changes in Membrane Phospholipid Asymmetry (pp. 131-142)
    Comfurius, Paul; Bevers, Edouard; Zwaal, Robert F. A.
12. Fluorescent Glycerolipid Probes: Synthesis and Use for Examining Intracellular Lipid Traficking (pp. 143-160)
    Sleight, Richard G.
13. Synthesis and Use of Spin-Labeled Lipids for Studies of the Transmembrane Movement of Phospholipids (pp. 161-176)
    Fellmann, Pierre; Zachowski, Alain; Devaux, Philippe E
14. Measurement of Membrane Fluidity and Membrane Fusion with Fluorescent Probes (pp. 177-188)
    Bashford, C. Lindsay
15. Extraction and Assay of Cyclic Nucleotides (pp. 189-198)
    Whitley, Guy St. J.
16. Analysis of G-Proteins Regulating Signal Transduction Pathways (pp. 199-212)
    Harnett, Margaret M.
17. Assay of Protein Kinases and Protein Phosphorylation (pp. 213-226)
    Harnett, Margaret M.
18. Analysis of Cellular Phosphoinositides and Phosphoinositols by Extraction and Simple Analytical Procedures (pp. 227-248)
    Bird, Ian M.
19. Analysis of Cellular Phosphoinositides and Phosphoinositols By High-Performance Liquid Chromatography (pp. 249-278)
    Bird, Ian M.
20. Cytosolib Free Calcium Measurements in Single Cells Using Calcium-Sensitive Fluorochromes (pp. 279-294)
    Zaidi, Mone; Alam, A S. M. Towhidul; Bax, Christopher; Shankar, Vijay; Bevis, Peter J. R; Huang, Christopher L. H.; Paxianas, Michael; Moonga, Baljit S.
21. Membrane Permeabilization with Bacterial Toxins (pp. 295-306)
    Bashford, C. Lindsay
22. Measurement of Ion Fluxes and pH Gradients Across Cell Membranes (pp. 307-324)
    Bashford, C. Lindsay
23. Ligand Binding and Processing: The Perfused Liver as a Model System (pp. 325-342)
    Renfrew, Carol A.; Casciola-Rosen, Livia A; Hubbard, Ann L.
24. The Binding of Protein-Ligands to Cell-Surface Receptors (pp. 343-350)
    Grant, David A. W.

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