Library Science

For both volumes: Expert investigators describe not only the classic methods, but also the many novel techniques they have perfected for the transfer of large DNAs into the cells of both microbes and animals via large-insert recombinant DNAs. Volume 1 presents readily reproducible techniques for library construction, physical mapping, and sequencing.. An accompanying volume, Volume 2: Functional Studies, provides a wide variety of methods and applications for functional analysis of the DNA-transformed organisms. Besides protocols, each chapter includes scientific reviews, software tools, database resources, genome sequencing strategies, and illustrative case studies.

Authors: Zhao, Shaying; Stodolsky, Marvin

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Table of Contents

1. Use of BAC End Sequences for SNP Discovery (pp. 001-006)
   Weil, Michael M.; Pershad, Rashmi; Wang, Ruoping; Zhao, Sheng
2. Exon Trapping for Positional Cloning and Fingerprinting (pp. 007-020)
   Wenderfer, Scott E.; Monaco, John J.
3. Isolation of CpG Islands From BAC Clones Using a Methyl-CpG Binding Column (pp. 021-038)
   Cross, Sally H.
4. BAC Microarray-Based Comparative Genomic Hybridization (pp. 039-056)
   Snijders, Antoine M.; Segraves, Richard; Blackwood, Stephanie; Pinkel, Daniel; Albertson, Donna G.
5. Large DNA Transformation in Plants (pp. 057-068)
   Choi, Sangdun
6. Retrofitting BACs With a Selectable Marker for Transfection (pp. 069-076)
   Wang, Zunde; Longacre, Angelika; Engler, Peter
7. BAC Modification Using a RecA Expressing Shuttle Vector System (pp. 077-088)
   Mise, Nathan; Avner, Philip
8. Bacterial Artificial Chromosome Engineering (pp. 089-106)
   Swaminathan, Srividya; Sharan, Shyam K.
9. ET Recombination: DNA Engineering Using Homologous Recombination in E. coli (pp. 107-122)
   Muyrers, Joep P. P.; Zhang, Youming; Benes, Vladmir; Testa, Giuseppe; Rientjes, Jeanette M. J.; Stewart, A. Francis
10. BAC Engineering for the Generation of ES Cell-Targeting Constructs and Mouse Transgenes (pp. 123-140)
    Testa, Giuseppe; Vintersten, Kristina; Zhang, Youming; Benes, Vladmir; Muyrers, Joep P. P.; Stewart, A. Francis
11. Microinjection of BAC DNA into the Pronuclei of Fertilized Mouse Oocytes (pp. 141-158)
    Vintersten, Kristina; Testa, Giuseppe; Stewart, A. Francis
12. Generation of BAC Transgenic Mice (pp. 159-182)
    Marshall, Vikki M.; Allison, Janette; Templeton, Tanya; Foote, Simon J.
13. BAC Rescue: A Tool for Functional Analysis of the Mouse Genome (pp. 183-198)
    Swing, Deborah A.; Sharan, Shyam K.
14. Herpesviruses: A Brief Overview (pp. 199-220)
    Ackermann, Mathias
15. Cloning of ß-Herpesvirus Genomes as Bacterial Artificial Chromosomes (pp. 221-240)
    Borst, Eva-Maria; Crnkovic-Mertens, Irena; Messerle, Martin
16. Construction of HSV-1 BACs and Their Use for Packaging of HSV-1-Based Amplicon Vectors (pp. 241-256)
    Heister, Thomas G. H.; Vogtlin, Andrea; Muller, Lars; Heid, Irma; Fraefel, Cornel
17. Mutagenesis of Viral BACs With Linear PCR Fragments (ET Recombination) (pp. 257-268)
    Wagner, Markus; Koszinowski, Ulrich H.
18. Mutagenesis of Herpesvirus BACs by Allele Replacement (pp. 269-280)
    Borst, Eva-Maria; Posfai, Gyorgy; Pogoda, Frank; Messerle, Martin
19. Herpesvirus Genome Mutagenesis by Transposon-Mediated Strategies (pp. 281-302)
    McGregor, Alistair; Schleiss, Mark R.
20. Protective DNA Vaccination by Particle Bombardment Using BAC DNA Containing a Replication-Competent, Packaging-Defective Genome of Herpes Simplex Virus Type I (pp. 303-308)
    Suter, Mark; Hefti, Hans Peter

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