Library Science

In Atherosclerosis: Experimental Methods and Protocols, Angela Drew and a panel of experts have assembled a comprehensive collection of traditional and cutting-edge techniques for investigating this disease and its possible treatments. Each readily reproducible method includes step-by-step instructions and practical information regarding sample collection, the choice of animal model system, experimental design, and practical data analysis techniques. Comprehensive and richly detailed, Atherosclerosis: Experimental Methods and Protocols enables all biomedical investigators to select those optimized techniques that may be most fruitfully used to study the development, progression, and treatment of atherosclerotic lesions today.

Authors: Drew, Angela F.

Table of Contents

1. Animal Models of Diet-Induced Atherosclerosis (pp. 1-6)
   Drew, Angela F.
2. Mechanical Injury Models: Balloon Catheter Injury to Rat Common Carotid Artery (pp. 7-13)
   Dilley, Rodney J.
3. Genetically Manipulated Models of Atherosclerosis in Mice (pp. 15-26)
   Xiao, Qing
4. Lipoprotein Isolation and Analysis from Serum by Preparative Ultracentrifugation (pp. 27-35)
   Wasan, Kishor M.; Cassidy, Shawn M.; Kennedy, Allison L.; Peteherych, Kathy D.
5. Separation of Plasma Lipoproteins in Self-Generated Gradients of Iodixanol (pp. 37-49)
   Higgins, Joan A.; Graham, John M.; Davies, Ian G.
6. Fractionation of Lipoprotein Subclasses in Self-Generated Gradients of Iodixanol (pp. 51-59)
   Graham, John M.; Griffin, Bruce A.; Davies, Ian G.; Higgins, Joan A.
7. Detecting Cholesteryl Ester Transfer Protein in Plasma (pp. 61-76)
   Ritsch, Andreas; Patsch, Josef R.
8. Determination of Plasma Homocysteine (pp. 77-103)
   Accinni, Roberto; Parodi, Oberdan
9. Assay for Serum Glycated Lipoproteins (pp. 105-111)
   Tanaka, Akira
10. Lipoprotein(a) Quantitation (pp. 113-122)
    Bender, Michael; Gross, Werner
11. Isolation and Characterization of Glycosaminoglycans from Human Atheromatous Vessels (pp. 123-136)
    Papakonstantinou, Eleni; Roth, Michael; Karakiulakis, George
12. Culture of Human Smooth Muscle Cells (pp. 137-146)
    Gallicchio, Marisa A.
13. Culture of Human Endothelial Cells (pp. 147-161)
    Gallicchio, Marisa A.
14. Assay for Cell Migration and Invasion of Vascular Smooth Muscle Cells (pp. 163-169)
    Karakiulakis, George; Papakonstantinou, Eleni; Roth, Michael
15. Collection and Processing of Arterial Specimens for Histological Analysis (pp. 171-175)
    Drew, Angela F.
16. Intravascular Ultrasound Imaging in the Quantitation of Atherosclerosis in Vivo (pp. 177-186)
    Manninen, Hannu I.
17. Identification of Cell Types and Quantification of Lesion Composition (pp. 187-194)
    Dilley, Rodney J.
18. Nonradioactive In Situ Hybridization in Atherosclerotic Tissue (pp. 195-206)
    Apostolopoulos, Jim
19. Evaluation of Angiogenesis (pp. 207-211)
    Silver, Marcy; Isner, Jeffrey M.
20. Evaluation of Cell Proliferation in Human Atherosclerotic Lesions (pp. 213-218)
    Andreeva, Elena R.; Orekhov, Alexander N.
21. Gene Transfer to the Vasculature: Historical Perspective and Implications for Future Research Objectives (pp. 219-231)
    George, Sarah J.; Baker, Andrew H.
22. A Pig Model of Vein Graft Disease: Applications for Potential Gene Therapies (pp. 233-243)
    Lloyd, Clinton T.; George, Sarah J.; Angelini, Gianni D.; Newby, Andrew C.; Baker, Andrew H.

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