Library Science

Experienced scientists describe-in an easily-followed format-their state-of-the-art techniques for evaluating antiviral compounds. The assays described include systems for investigating drugs used against herpesviruses, hepatitis viruses, human immunodeficiency viruses, human papillomaviruses, and influenza viruses. These well-tested methods range from cellular assays to some of the most up-to-date molecular approaches for identifying compounds that are active against viral enzymes and the development of viral resistance against drugs currently in use. Timely and comprehensive, Antiviral Methods and Protocols offers today’s researchers in academia, clinical departments, and the pharmaceutical industry the powerful, reproducible, and novel methods needed to evaluate compounds effective against both acute and chronic infections. Ed. Schinazi, Raymond F.

Table of Contents

1. Changing Methods for Discovering Antiviral Drugs (pp. 1-12)
   Jones, Philip S.
2. Laboratory Safety Considerations (pp. 13-26)
   Feorino, Paul M.; Williamson, John D.; Schinazi, Raymond F.
3. Routine Quality Control Testing of Cell Cultures: Detection of Mycoplasma (pp. 27-40)
   Stacey, Alison; Stacey, Glyn
4. Hep AD38 Assay: A High-Throughput, Cell-Based Screen for the Evaluation of Compounds Against Hepatitis B Virus (pp. 43-50)
   King, Robert W.; Ladner, Stephanie K.
5. Hepatitis B Virus Cell Culture Assays for Antiviral Activity (pp. 51-68)
   Schmidt, Karen; Korba, Brent
6. A Rapid Microtiter Assay for Duck Hepatitis Virus Reverse Transcriptase (pp. 69-76)
   Otto, Michael J.; Whitaker, Richard A.
7. Assays for Measuring Hepadnaviral Supercoiled DNA (pp. 77-86)
   Locarnini, Stephen A.; Civitico, Gilda M.
8. Assays for Hepatitis B Virus DNA- and RNA-Dependent DNA Polymerase Activities (pp. 87-96)
   Shaw, Timothy; Locarnini, Stephen A.
9. Development of a Hepatitis C Virus RNA Helicase High Throughput Assay (pp. 97-116)
   Kwong, Ann D.; Risano, Christine
10. Determination of Viral Infectivity: HSV, VZV, and CMV (pp. 119-128)
    Harper, David R.
11. Antiviral Screening Assays for Human Herpesviruses 6 and 7 (pp. 129-138)
    Black, Jodi B.; Pellett, Philip E.
12. Strategies for Evaluation of Antiviral Agents Against Epstein-Barr Virus in Culture (pp. 139-150)
    Lin, Jung-Chung
13. Monitoring Drug Resistance for Herpesviruses (pp. 151-170)
    Andrei, Graciela; Fiten, Pierre; De Clercq, Erik; Snoeck, Robert; Opdenakker, Ghislain
14. Herpesvirus Protease Assays (pp. 171-182)
    Ertl, Peter; Russell, Linda; Angier, Jane
15. Testing Compounds for Antiviral Activity in Cell Cultures Infected with HIV (pp. 185-200)
    Devine, Donna; Mathews, Naomi; Kinchington, Derek
16. Evaluation of Compounds That Prevent Reactivation of HIV-1 in OM-10.1 Cells (pp. 201-206)
    Butera, Salvatore T.
17. Assessment of Activity of Topical Virucidal Agents (pp. 207-212)
    O???Connor, Timothy
18. Assays That Measure Selective Killing of Virus-Infected Cells (pp. 213-222)
    Randall, Sharon
19. Evaluating Clinical Isolates for Their Phenotypic and Genotypic Resistance Against Anti-HIV Drugs (pp. 223-258)
    Vandamme, Anne-Mieke; Witvrouw, Myriam; Pannecouque, Christophe; Balzarini, Jan; Van Laethem, Kristel; Schmit, Jean-Claude; Desmyter, Jan; De Clercq, Erik
20. Line Probe Assay for Detecting Mutations in HIV-1 Reverse Transcriptase (pp. 259-268)
    Stuyver, Lieven; Wyseur, Ann; Verpooten, Gonda; Rossau, Rudi
21. RT-PCR for Amplification of Specific Fragments of HIV-1 Genome (pp. 269-282)
    de Bethune, Marie-Pierre; Hertogs, Kurt
22. Evaluation of Compounds Against Recombinant HIV Reverse Transcriptase (pp. 283-290)
    Balzarini, Jan
23. Ultrasensitive Detection of Reverse Transcriptase Activity by the Amp-RT Assay: Applications to the Measurement of Virus Loads and Phenotypic Drug Resistance (pp. 291-300)
    Lerma, J. Gerardo Garcia; Heneine, Walid
24. Reverse Transcriptase Assay Based on Product Enhancement for Assessing the Drug Susceptibility of Retroviruses (pp. 301-312)
    Boni, Jurg; Schupbach, Jorg
25. Identifying and Characterizing HIV Protease Inhibitors (pp. 313-326)
    Furfine, Eric S.
26. Retroviral Integrase: A Novel Target in Antiviral Drug Development and Basic In Vitro Assays with the Purified Enzyme (pp. 327-338)
    Mazumder, Abhijit; Neamati, Nouri; Sunder, Sanjay; Owen, Joshua; Pommier, Yves
27. Papillomavirus DNA Replication (pp. 341-360)
    Ferran, Maureen C.; McBride, Alison A.
28. Quantitative Immunodetection of Viral Oncoprotein Levels in Human Papillomavirus???Transformed Human Cells (pp. 361-364)
    Johnson, Jeffrey A.; Gangemi, J. David
29. Biochemical Methods for the Characterization of Influenza Viruses with Reduced Sensitivity to 4-Guanidino-Neu5Ac2en (pp. 367-374)
    Bethell, Richard C.; Hart, Graham J.; Blick, Tony J.; Sahasrabudhe, Anjali; McKimm-Breschkin, Jennifer L.
30. Virological Methods for the Generation and Characterization of Influenza Viruses with Reduced Sensitivity to 4-Guanidino-Neu5Ac2en (pp. 375-382)
    McKimm-Breschkin, Jennifer L.; Sahasrabudhe, Anjali; Blick, Tony J.; Jowett, Amanda J.; Bethell, Richard C.

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