Library Science

Stephen Gillespie and a panel of leading clinical and diagnostic microbiologists describe a series of detailed molecular and physical methods designed to study the growing problem of antibiotic resistance, as well as facilitate new antibiotic research programs for its effective redress. The techniques range widely from those that provide rapid diagnosis via DNA amplification and phage display, to those for plotting the transmission of resistant organisms and investigating their epidemiology. The methods are readily adaptable to a wide range of resistant bacterial organisms. Practical and wide-ranging, Antibiotic Resistance: Methods and Protocols provides a collection of indispensable techniques not only for illuminating the basic biology of antimicrobial resistance, but also for developing and implementing new diagnostic and epidemiological tools.

Authors: Ed. Gillespie, Stephen H.

  Download Book (2844Kb PDF)

Table of Contents

1. Multiplex Polymerase Chain Reaction Detection of vanA, vanB, vanC-1, and vanC-2/3 Genes in Enterococci (pp. 3-11)
   Patel, Robin; Uhl, Jim R.; Cockerill, Franklin R.
2. Drug Susceptibility of Mycobacterium tuberculosis Through the Mycolic Acid Index (pp. 13-19)
   Viader-Salvado, Jose M.; Guerrero-Olazaran, Martha; Garza-Gonzalez, Elvira; Tijerina-Menchaca, Rolando
3. Micro-Well Phage Replication Assay for Screening Mycobacteria for Resistance to Rifampin and Streptomycin (pp. 21-30)
   McNerney, Ruth
4. Application of SSCP to Identification of Resistance Mutations (pp. 31-37)
   McHugh, Timothy D.
5. Quantitative, Single-Tube, Nested, PCR (QSTN-PCR) for Determining the Antibiotic Susceptibility of Mycobacterium tuberculosis (pp. 39-54)
   Jou, Nainn-Tsyr; Liebling, Michael R.
6. Rapid Rifamycin Susceptibility Testing of Small-Volume Mycrobacterium tuberculosis Cultures by Detection of Precursor rRNA (pp. 55-63)
   Brabant, William H.; Cangelosi, Gerard A.
7. Detection of Penicillin Resistance in Streptococcus pneumoniae by a Seminested PCR Strategy (pp. 65-75)
   du Plessis, Mignon; Smith, Anthony M.; Klugman, Keith P.
8. Diagnosis of Penicillin Resistance by PCR-RFLP (pp. 77-84)
   Whiting, Gail C.
9. Detection Methods of Glycopeptide-Resistant Staphylococcus aureus I: Susceptibility Testing (pp. 85-91)
   Hanaki, Hedeaki; Hiramatsu, Keiichi
10. Detection Methods for Glycopeptide-Resistant Staphylococcus aureus II: Cell Wall Analysis (pp. 93-101)
    Hanaki, Hedeaki; Hiramatsu, Keiichi
11. Multiplex PCR for the Rapid Simultaneous Speciation and Detection of Methicillin-Resistance and Genes Encoding Toxin Production in Staphylococcus aureus (pp. 103-112)
    Jones, Mark E.; Kohrer, Karl; Schmitz, Franz-Josef
12. Chromogenic Detection of Aminoglycoside Phosphotransferases (pp. 113-117)
    Amoroso, Ana M.; Gutkind, Gabriel O.
13. Quantification of M. tuberculosis DNA in Sputum During the Treatment of Pulmonary Tuberculosis (pp. 121-131)
    DesJardin, Lucy E.; Hehman, Gery L.
14. Isolation of M. tuberculosis RNA from Sputum (pp. 133-139)
    DesJardin, Lucy E.
15. Detection of Viable Mycobacterium tuberculosis by Reverse Transcriptase-Strand Displacement Amplification of mRNA (pp. 141-155)
    Hellyer, Tobin J.
16. BOX PCR Fingerprinting for Molecular Typing of Streptococcus pneumoniae (pp. 159-168)
    van Belkum, Alex; Hermans, Peter W. M.
17. Restriction Fragment End Labeling Analysis: High-Resolution Genomic Typing of Streptococcus pneumoniae Isolates (pp. 169-179)
    Hermans, Peter W. M.; Sluijter, Marcel; van Belkum, Alex
18. Pulse-field Gel Electrophoresis for Epidemiological Studies of Streptococcus pneumoniae (pp. 181-187)
    Gasc, Anne-Marie
19. Outer Membrane Profiles of Clonally Related Klebsiella pneumoniae (pp. 189-197)
    Benedi, Vicente J.; Martinez-Martinez, Luis
20. Atomic Force Microscopy: Theory and Practice in Bacteria Morphostructural Analysis (pp. 199-207)
    Braga, Pier Carlo; Ricci, Davide
21. Assessing the Activity of Bacterial Multidrug Efflux Pumps (pp. 211-214)
    Poole, Keith; Srikumar, Ramakrishnan
22. The Use of a Continuous Culture System to Study the Antimicrobial Susceptibility of Bacteria in Biofilm (pp. 215-225)
    Struthers, J. Keith
23. Estimation of Mutation Rates in Antibiotic Research (pp. 227-232)
    Billington, Owen J.; Gillespie, Stephen H.
24. In Vitro Assessment of the Fitness of Resistant M. tuberculosis Bacteria by Competition Assay (pp. 233-236)
    Gillespie, Stephen H.
25. Purification of DNA Topoisomerases and Inhibition by Fluoroquinolones (pp. 237-244)
    George, John T.; Morrissey, Ian
26. Site-Directed Mutagenesis to Determine Structure Function Relationships in Streptococcus pneumoniae Penicillin-Binding Protein Genes (pp. 245-264)
    Barcus, Victoria A.; Dowson, Christopher G.
27. Detection of Low Affinity Penicillin-Binding Protein Variants in Streptococcus pneumoniae (pp. 265-272)
    Hakenbeck, Regine
28. Mobilization of Transposons: Rationale and Techniques for Detection (pp. 275-284)
    Rice, Louis B.

This entry was posted on Monday, December 11th, 2006 at 4:09 pm and is filed under Molecular Medicine. You can follow any responses to this entry through the RSS 2.0 feed. You can leave a response, or trackback from your own site.