Library Science

This superb collection of well-tested protocols for the electroporation of proteins and DNA into insect, fish, and mammalian cells also includes proven animal cell electrofusion techniques for studies of somatic cell genetics and development, and for generating monoclonal antibodies. It is distinguished by its coverage of important model cell types from many organisms and tissue types and its detailed instructions for the growth and preparation of specific cells to achieve optimum transfection. Its extensive reference lists, citations of alternative transfer methods, advice on pitfalls to avoid, and descriptions of expected results ensure readily reproducible results even for beginners.

Authors: Nickoloff, Jac A.

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Table of Contents

1. Electroporation Theory: Concepts and Mechanisms (pp. 3-28)
   Weaver, James C.
2. Effects of Pulse Length and Strength on Electroporation Efficiency (pp. 29-40)
   Hui, Sek Wen
3. Instrumentation (pp. 41-60)
   Hofmann, Gunter A.
4. The Introduction of Proteins into Mammalian Cells by Electroporation (pp. 63-72)
   Morgan, William F.; Day, Joseph P.
5. Electroporation of Antigen-Presenting Cells for T-Cell Recognition and Cytotoxic T-Lymphocyte Priming (pp. 73-82)
   Chen, Weisan; McCluskey, James
6. Electroporation of Antibodies into Mammalian Cells (pp. 83-92)
   Campbell, Paul L.; McCluskey, James; Yeo, Jing Ping; Toh, Ban-Hock
7. Electroporation of Adherent Cells In Situ for the Introduction of Nonpermeant Molecules (pp. 93-114)
   Raptis, Leda H.; Firth, Kevin L.; Brownell, Heather L.; Todd, Andrea; Simon, W. Craig; Bennett, Brian M.; MacKenzie, Leslie W.; Zannis-Hadjopoulos, Maria
8. Electrotransformation of Chinese Hamster Ovary Cells (pp. 115-122)
   Taghian, Danielle Gioioso; Nickoloff, Jac A.
9. Electroporation of Rat Pituitary Cells (pp. 123-132)
   Paulssen, Ruth H.; Paulssen, Eyvind J.; Gautvik, Kaare M.
10. Electroporation of Plasmid DNA into Normal Human Fibroblasts (pp. 133-140)
    Ray, F. Andrew
11. Electroporation-Mediated Gene Transfer into Hepatocytes (pp. 141-150)
    Le Cam, Alphonse
12. Electroporation of Human Lymphoblastoid Cells (pp. 151-160)
    Xia, Fen; Liber, Howard L.
13. The Use of Electroporated Bovine Spermatozoa to Transfer Foreign DNA into Oocytes (pp. 161-166)
    Gagne, Marc; Pothier, Francois; Sirard, Marc-Andre
14. Electroporation of Embryonic Stem Cells for Generating Transgenic Mice and Studying In Vitro Differentiation (pp. 167-184)
    Mudgett, John S.; Livelli, Thomas J.
15. Electrotransfection with ??°Intracellular??² Buffer (pp. 185-198)
    Hoff, Maurice J. B. van den; Christoffels, Vincent M.; Labruyere, Wil T.; Moorman, Antoon F. M.; Lamers, Wouter H.
16. Effect of Cis-Located Human Satellite DNA on Electroporation Efficiency (pp. 199-210)
    Saint-Dic, Djenann; DuBow, Michael S.
17. Quantitation of Transient Gene Expression (pp. 211-224)
    Showe, Michael K.; Showe, Louise C.
18. Stable Integration of Vectors at High Copy Number for High-Level Expression in Animal Cells (pp. 225-238)
    Barsoum, James
19. Electroporation of Drosophila Embryos (pp. 239-244)
    Kamdar, K. Puloma; Wagner, Thao N.; Finnerty, Victoria
20. Transformation of Fish Cells and Embryos (pp. 245-252)
    Inoue, Koji; Hata, Jun-ichiro; Yamashita, Shinya
21. Electroporation of Cardiac Cells (pp. 253-272)
    Tung, Leslie
22. Electroporation for Gene Therapy (pp. 273-280)
    Matthews, Kathryn E.; Dev, Sukhendu B.; Toneguzzo, Frances; Keating, Armand
23. Electrofusion of Mammalian Cells (pp. 283-294)
    White, Kenneth L.
24. Stabilizing Antibody Secretion of Human Epstein Barr Virus-Activated B-Lymphocytes with Hybridoma Formation by Electrofusion (pp. 295-308)
    Perkins, Susan; Foung, Steven K H.
25. Electrofusion of Mammalian Oocytes and Embryonic Cells (pp. 309-316)
    Fulka, Josef; Moor, Robert M.; Fulka, Josef
26. Nuclear Transfer in Bovine Embryos (pp. 317-330)
    Iritani, Akira; Mitani, Tasuku
27. Electrofusion of Mouse Embryos to Produce Tetraploids (pp. 331-340)
    Petzoldt, Ulrich
28. Spectrofluorometric Assay for Cell-Tissue Electrofusion (pp. 341-354)
    Heller, Richard
29. Cytometric Detection and Quantitation of Cell???Cell Electrofusion Products (pp. 355-364)
    Jaroszeski, Mark J.; Gilbert, Richard; Heller, Richard

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