Library Science

Donna Wang and a panel of hands-on experts describe in step-by-step detail all the major molecular, biochemical, and functional techniques now used in probing the renin???angiotensin system. These range from the use of embryonic gene targeting to create of mutant strains, to the functional investigation of components of the renin???angiotensin system. Comprehensive and highly practical, Angiotensin Protocols translates expert knowledge into readily reproducible methods that enable all biomedical investigators successfully to explore the role of angiotensin in health and disease today.

Authors: Wang, Donna H.

Table of Contents

1. Historical Perspective of the Renin-Angiotensin System (pp. 3-21)
   Hall, John E.
2. Three Angiotensin Paradigms in One Patient: Etiology of Hypertension, Glomerular Hemodynamics, and Long-Term Glomerular Protection (pp. 23-37)
   Laffer, Cheryl L.; Elijovich, Fernando
3. Production and Use of Chimeric Mice (pp. 41-52)
   Takaya, Junji; Matsusaka, Taiji; Ichikawa, Iekuni
4. Genetic Manipulation of the Renin-Angiotensin System Using Cre-loxP-Recombinase (pp. 53-65)
   Sigmund, Curt D.; Stec, David E.
5. Systemic Delivery of a Transgene in Intact Animals by a Retroviral Vector (pp. 67-81)
   Katovich, Michael J.; Wang, Hong-Wei; Gelband, Craig H.; Raizada, Mohan K.
6. Antisense Inhibition of the Renin-Angiotensin System (pp. 83-104)
   Mohuczy, Dagmara; Phillips, M. Ian
7. Analysis of Transcriptional Control Mechanisms I: Techniques for Characterization of cis -Regulatory Elements (pp. 107-125)
   Sherman, Christopher T.; Brasier, Allan R.
8. Analysis of Transcriptional Control Mechanisms II: Techniques for Characterization of trans -Acting Factors (pp. 127-150)
   Brasier, Allan R.; Sherman, Christopher T.; Jamaluddin, Mohammad
9. Analysis of Cytosolic Proteins that Bind to the 5??? Leader Sequence of the Angiotensin AT1 Receptor by RNA Electromobility Shift Assay (pp. 151-170)
   Wu, Zheng; Krishnamurthi, Kamakshi; Mok, Koby; Sandberg, Kathryn
10. In Vitro Translation of the Angiotensin AT1 Receptor in Wheat-Germ Extracts (pp. 171-192)
    Ji, Hong; Krishnamurthi, Kamakshi; Wu, Zheng; Sandberg, Kathryn
11. Quantitation of Angiotensin II Receptors by Competitive Reverse-Transcription Polymerase Chain Reaction (pp. 195-204)
    Wu, Wei; Yao, Aqing; Wang, Donna H.
12. Analysis of RNA by Northern-Blot Hybridization (pp. 205-213)
    Gallagher, Patricia E.; Diz, Debra I.
13. Use of the Ribonuclease Protection Assay for the Analysis and Characterization of Target mRNAs (pp. 215-222)
    Supowit, Scott C.; DiPette, Donald J.
14. In Situ Hybridization (pp. 223-233)
    Runge, Susan Riggs; Hu, Zhaoyong; Runge, Marschall S.
15. Immunohistochemical Detection of Angiotensin II Receptor (pp. 237-244)
    Wang, Zhi-Qin; Carey, Robert M.
16. Western Blotting (pp. 245-256)
    Madamanchi, Nageswara R.; Runge, Marschall S.
17. Quantification of Angiotensin-Converting Enzyme (ACE) Activity (pp. 257-266)
    Meng, Qing Cheng; Berecek, Kathleen H.
18. Quantification of Angiotensin Peptides (pp. 267-273)
    Meng, Qing Cheng; Berecek, Kathleen H.
19. Radiolabeling of Angiotensin Peptides (pp. 275-295)
    Speth, Robert C.; Harding, Joseph W.
20. Assays for Radiolabel-Photoaffinity Labeling of Angiotensin Receptors (pp. 297-303)
    Perodin, Jacqueline; Boucard, Antony A.; Leduc, Richard; Escher, Emanuel; Guillemette, Gaetan
21. Radioligand Binding Assay (pp. 305-314)
    Thibault, Gaetan; Schiffrin, Ernesto L.
22. Autoradiographic Localization and Quantification of Components of the Renin-Angiotensin System in Tissues (pp. 315-337)
    Chai, Siew Yeen; Allen, Andrew M.; Zhuo, Jialong; Moeller, Ingrid; Mendelsohn, Frederick A. O.
23. Measurement of Intracellular Free Calcium Ion Concentration in Vascular Smooth Muscle Cells: Fluorescence Imaging of Cytosolic Calcium (pp. 341-354)
    Touyz, Rhian M.; Schiffrin, Ernesto L.
24. Measurement of Phospholipase D Activation in Vascular Smooth Muscle Cells (pp. 355-362)
    Touyz, Rhian M.; Schiffrin, Ernesto L.
25. Measurement of Cyclic AMP and Cyclic GMP in Xenopus Oocytes Stimulated with Angiotensin II and Atrial Natriuretic Factor (pp. 363-377)
    Ji, Hong
26. Measurement of Nitric Oxide Formation (pp. 379-389)
    Thorup, Christian; Moore, Leon C.
27. Measuring Renin Secretion from Juxtaglomerular Cells (pp. 391-404)
    Kurtz, Armin; Schweda, Frank
28. Measurement of Regional Blood Flow in the Kidney Using Laser-Doppler Flowmetry (pp. 407-426)
    Roman, Richard J.; Mattson, David L.; Cowley, Allen W.
29. Measurement of Renal Tubular Angiotensin II (pp. 427-434)
    Imig, John D.; Navar, L. Gabriel
30. The In Vitro Blood-Perfused Juxtamedullary Nephron Technique (pp. 435-449)
    Inscho, Edward W.
31. In Vitro Proximal Tubule Perfusion Preparation (pp. 451-470)
    Garvin, Jeffrey L.; Harris, Peter J.
32. Isolated Resistance Artery Preparation (pp. 471-487)
    Wesselman, Jos P. M.; Ratz, Paul H.; Prewitt, Russell L.
33. Measurement of Vascular Density (pp. 489-496)
    Greene, Andrew S.; Rieder, Mark J.
34. Functional Studies of the Cerebral Circulation (pp. 497-514)
    Lombard, Julian H.

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