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A comprehensive collection of key techniques for assessing the formation of new blood vessels in both whole tissues and cell culture. These readily reproducible methods range from low-cost in vitro assays to cutting-edge in vivo models, such as the dorsal window chamber, which demands high levels of surgical skill as well as relatively expensive, custom-made equipment. Timely and highly practical, Angiogenesis Protocols is a gold-standard resource for today’s angiogenesis investigator, providing easy access to a wide variety of proven assays suitable for laboratories with greatly differing technical expertise, materials, and financial resources.

Authors: Ed. J. C. Murray

Table of Contents

1. Therapeutic Inhibition of Angiogenesis (pp. 003-026)
   Zhang, Hua-Tang; Bicknell, Roy
2. Microscopic Assessment of Angiogenesis in Tumors (pp. 029-046)
   Fox, Stephen B
3. In Vivo Matrigel Migration and Angiogenesis Assays (pp. 047-052)
   Malinda, Katherine M
4. Alginate Microbead Release Assay of Angiogenesis (pp. 053-057)
   Ko, Clifford Y; Dixit, Vivek; Shaw, William W; Gitnick, Gary
5. Disc Angiogenesis Assay (pp. 059-075)
   Allison, Anthony C; Fajardo, Luis-F
6. Sponge Implant Model of Angiogenesis (pp. 077-086)
   Andrade, Silvia P
7. Hollow Fiber Assay for Tumor Angiogenesis (pp. 087-093)
   Phillips, Roger M; Bibby, Michael C
8. Dorsal Skinfold Chamber Preparation in Mice: Studying Angiogenesis by Intravital Microscopy (pp. 095-105)
   Sckell, Axel; Leunig, Michael
9. Angiogenesis Assays Using Chick Chorioallantoic Membrane (pp. 107-129)
   West, David C; Thompson, W Douglas; Sells, Paula G; Burbridge, Mike F
10. Corneal Assay for Angiogenesis (pp. 131-142)
    Ziche, Marina
11. Collagen Gel Assay for Angiogenesis: Induction of Endothelial Cell Sprouting (pp. 145-162)
    Schor, Ana M; Ellis, Ian; Schor, Seth L
12. Chemotaxis and Chemokinesis in 3D Macromolecular Matrices: Relevance to Angiogenesis (pp. 163-183)
    Schor, Ana M; Ellis, Ian; Schor, Seth L
13. Rat Aortic Ring: 3D Model of Angiogenesis In Vitro (pp. 185-204)
    Burbridge, Mike F; West, David C
14. In Vitro Matrigel Angiogenesis Assays (pp. 205-209)
    Ponce, M Lourdes
15. Microvessel Endothelial Cells from Human Adipose Tissues: Isolation, Identification, and Culture (pp. 213-226)
    Hewett, Peter W
16. Transfection and Transduction of Primary Human Endothelial Cells (pp. 227-235)
    Martin, Stewart G
17. Vascular Smooth Muscle Cells: Isolation, Culture, and Characterization (pp. 237-245)
    Siow, Richard C M; Pearson, Jeremy D
18. Bovine Retinal Microvascular Pericytes: Isolation, Propagation, and Identification (pp. 247-263)
    Nayak, Ramesh C; Herman, Ira M

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