Alzheimer’s Disease: Methods and Protocols
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A panel of highly skilled investigators describe in detail their state-of-the-art biochemical, cell biological, and molecular biological techniques for studying the molecular basis of Alzheimer’s disease. These readily reproducible, step-by-step methods focus on work with the amyloid precursor protein and the amyloidogenic peptide A?, but also include-with the recent identification of presenilin proteins-techniques for determining the structure and biological function of these proteins. In addition, there are chapters covering the tau protein and its role in Alzheimer’s disease, as well as an introductory discussion of the history of the disease, its genetic basis, and the currently available and possible future therapeutic agents. Cutting-edge and wide ranging, Alzheimer’s Disease: Methods and Protocols provides ready access to proven, reproducible methods for elucidating the molecular basis of this most common senile dementia. Ed. Hooper, Nigel
Table of Contents
- Introduction to Alzheimer???s Disease (pp. 1-22)
Allsop, David - The Genetics of Alzheimer???s Disease (pp. 23-44)
Brindle, Nick; St. George-Hyslop, Peter - Advances in Methodology and Current Prospects for Primary Drug Therapies for Alzheimer???s Disease (pp. 45-62)
Knopman, David S. - Production and Functional Assays of Recombinant Secreted Amyloid Precursor Protein (APP) (sAPPa) (pp. 63-78)
Barger, Steven W. - Quantifying A?В1-40 and A?В1-42 Using Sandwich-ELISA (pp. 79-90)
Skovronsky, Daniel M.; Wang, Jun; Lee, Virginia M.-Y.; Doms, Robert W. - Electrophoretic Separation and Immunoblotting of A?В1-40 and A?В1-42 (pp. 91-100)
Staufenbiel, Matthias; Paganetti, Paolo A. - A?В-Induced Proinflammatory Cytokine Release from Differentiated Human THP-1 Monocytes (pp. 101-112)
Brunden, Kurt R.; Kocsis-Angle, June; Embury, Paula; Yates, Stephen L. - Effects of the ?В-Amyloid Peptide on Membrane Ion Permeability (pp. 113-138)
Pearson, Hugh A. - Analysis of ?В-Amyloid Peptide Degradation In Vitro (pp. 139-148)
Cordell, Barbara; Naidu, Asha - Posttranslational Modifications of Amyloid Precursor Protein: Ectodomain Phosphorylation and Sulfation (pp. 149-168)
Walter, Jochen; Haass, Christian - Posttranslational Modifications of the Amyloid Precursor Protein: Glycosylation (pp. 169-190)
Liu, Chen; Rozmyslowicz, Tomasz; Stwora-Wojczyk, Magda; Wojczyk, Boguslaw; Spitalnik, Steven L. - Using an Amyloid Precursor Protein (APP) Reporter to Characterize a-Secretase (pp. 191-202)
Roberts, Susan Boseman - Inhibition of a-Secretase by Zinc Metalloproteinase Inhibitors (pp. 203-216)
Parvathy, S.; Turner, Anthony J.; Hooper, Nigel M. - Development of Neoepitope Antibodies Against the ?В-Secretase Cleavage Site in the Amyloid Precursor Protein (pp. 217-228)
Gray, Carol W.; Karran, Eric H. - ?В-Secretase: Tissue Culture Studies of Sequence Specificity, Inhibitors, and Candidate Enzymes (pp. 229-238)
Citron, Martin - Using y-Secretase Inhibitors to Distinguish the Generation of the A?В Peptides Terminating at Val-40 and Ala-42 (pp. 239-248)
Paganetti, Paolo A.; Staufenbiel, Matthias - Designing Animal Models of Alzheimer???s Disease with Amyloid Precursor Protein (APP) Transgenes (pp. 249-270)
Loring, Jeanne F. - Phosphorylation of Amyloid Precursor Protein (APP) Family Proteins (pp. 271-282)
Suzuki, Toshiharu; Ando, Kanae; Iijima, Ko-ichi; Oguchi, Shinobu; Takeda, Shizu - Determining the Transmembrane Topology of the Presenilins (pp. 283-296)
Thinakaran, Gopal; Doan, Andrew - Normal Proteolytic Processing of the Presenilins (pp. 297-308)
Hartmann, Henrike; Yankner, Bruce A. - Apoptotic Proteolytic Cleavage of the Presenilins by Caspases (pp. 309-316)
Kim, Tae-Wan - The Phosphorylation of Presenilin Proteins (pp. 317-332)
Walter, Jochen - Interaction of the Presenilins with the Amyloid Precursor Protein (APP) (pp. 333-344)
Weidemann, Andreas; Paliga, Krzysztof; Durrwang, Ulrike; Reinhard, Friedrich; Zhang, Dai; Sandbrink, Rupert; Evin, Genevieve; Masters, Colin L.; Beyreuther, Konrad - Distribution of Presenilins and Amyloid Precursor Protein (APP) in Detergent-Insoluble Membrane Domains (pp. 345-360)
Parkin, Edward T.; Turner, Anthony J.; Hooper, Nigel M. - Characterization and Use of Monoclonal Antibodies to Tau and Paired Helical Filament Tau (pp. 361-374)
Davies, Peter - Tau Phosphorylation Both In Vitro and in Cells (pp. 375-394)
Reynolds, C. Hugh; Gibb, Graham M.; Lovestone, Simon - Transglutaminase-Catalyzed Formation of Alzheimer-Like Insoluble Complexes from Recombinant Tau (pp. 395-404)
Balin, Brian J.; Appelt, Denah M.