Library Science

Agrobacterium Protocols offers beginning and experienced researchers the most comprehensive collection of step-by-step protocols for the genetic manipulation of plants using Agrobacterium. The topics range from the maintenance of bacterial culture collections to aspects of the metabolism and physiology of transformed tissues and transgenic plants. Drawing on the work of leading scientists from laboratories around the world, Agrobacterium Protocols provides a wealth of techniques for introducing specific DNA sequences into target plant species and discusses the environmental implications of genetically engineered plants. Its detailed procedures will facilitate rapid transfer of advanced techniques to other laboratories and their exploitation in fundamental and applied plant biology.

Authors: O???Hare, Sheila; Atterwill, Christopher K.

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Table of Contents

1. Growth and Storage of Agrobacterium (pp. 1-8)
   van Asma, Fraukje M.
2. Simple Cultural Tests for Identification of Agrobacterium Biovars (pp. 9-14)
   Bouzar, Hacene; Jones, Jeffrey B.; Bishop, Andrew L.
3. Agrobacterium Virulence (pp. 15-28)
   Gartland, Jill S.
4. Agrobacterium tumefaciens Chemotaxis Protocols (pp. 29-36)
   Shaw, Charles H.
5. Effect of Acetosyringone on Growth and Oncogenic Potential of Agrobacterium tumefaciens (pp. 37-46)
   Dion, Patrice; Belanger, Christian; Xu, Dong; Mohammadi, Mahmood
6. Binary Ti Plasmid Vectors (pp. 47-58)
   An, Gynheung
7. Leaf Disk Transformation (pp. 59-70)
   Curtis, Ian S.; Davey, Michael R.; Power, J. Brian
8. Agrobacterium-Mediated Transformation of Protoplasts from Oilseed Rape (Brassica napus L.) (pp. 71-78)
   Thomzik, Jurgen E.
9. Agrobacterium-Mediated Transformation of Stem Disks from Oilseed Rape (Brassica napus L.) (pp. 79-86)
   Thomzik, Jurgen E.
10. Peanut Transformation (pp. 87-100)
    Mansur, Elisabeth; Lacorte, Cristiano; Krul, William R.
11. Transformation of Soybean (Glycine max) via Agrobacterium tumefaciens and Analysis of Transformed Plants (pp. 101-120)
    Chee, Paula P.; Slightom, Jerry L.
12. Agrobacterium-Mediated Transformation of Potato Genotypes (pp. 121-128)
    Kumar, Amar
13. Agrobacterium-Mediated Transformation of Soft Fruit Rubus, Ribes, and Fragaria (pp. 129-134)
    Graham, Julie; McNicol, Ronnie J.; Kumar, Amar
14. High-Frequency and Efficient Agrobacterium-Mediated Transformation of Arabidopsis thaliana Ecotypes ??°C24??² and ??°Landsberg erecta??² Using Agrobacterium tumefaciens (pp. 135-148)
    Barghchi, Mehdi
15. Transformation Protocols for Broadleaved Trees (pp. 149-166)
    Fenning, Trevor M.; Gartland, Kevan M. A.
16. Agrobacterium-Mediated Antibiotic Resistance for Selection of Somatic Hybrids: The Genus Lycopersicon as a Model System (pp. 167-184)
    Davey, Michael R.; Patil, Rajendra S.; Lowe, Kenneth C.; Power, John B.
17. Histochemical GUS Analysis (pp. 185-194)
    Vitha, Stanislav; Benes, Karel; Phillips, Julian P.; Gartland, Kevan M. A.
18. Fluorometric GUS Analysis for Transformed Plant Material (pp. 195-200)
    Gartland, Kevan M. A.; Phillips, Julian P.; Vitha, Stanislav; Benes, Karel
19. The Detection of Neomycin Phosphotransferase Activity in Plant Extracts (pp. 201-206)
    Scheid, Ortrun Mittelsten; Neuhaus-Url, Gabriele
20. The Plant Oncogenes rolA, B, and C from Agrobacterium rhizogenes: Effects on Morphology, Development, and Hormone Metabolism (pp. 207-222)
    Michael, Tony; Spena, Angelo
21. Quantifying Polyamines in Agrobacterium rhizogenes Strains and in Ri Plasmid Transformed Cells (pp. 223-236)
    Bagni, Nello; Mengoli, Marisa; Matsuzaki, Shigeru
22. IAA Analysis in Transgenic Plants (pp. 237-244)
    Hall, John F.; Brown, Sarah J.; Gartland, Kevan M. A.
23. Quantifying Phytohormones in Transformed Plants (pp. 245-262)
    Prinsen, Els; Redig, Pascale; Strnad, Miroslav; Galis, Ivan; Van Dongen, Walter; Van Onckelen, Henri
24. Manipulating Photosynthesis in Transgenic Plants (pp. 263-280)
    Knight, Jacqueline S.; Madueno, Francisco; Barnes, Simon A.; Gray, John C.
25. Gene Activation by T-DNA Tagging (pp. 281-294)
    Fritze, Klaus; Walden, Richard
26. Assessing Cadmium Partitioning in Transgenic Plants (pp. 295-308)
    Wagner, George J.
27. Overexpression of Chloroplastic Cu/Zn Superoxide Dismutase in Plants (pp. 309-324)
    Allen, Randy D.
28. Agroinfection (pp. 325-342)
    Grimsley, Nigel
29. T-DNA Transfer to Maize Plants (pp. 343-350)
    Shen, Wen-Hui; Escudero, Jesus; Hohn, Barbara
30. Use of Cosmid Libraries in Plant Transformations (pp. 351-368)
    Ma, Hong
31. Regulation of Agrobacterium Gene Manipulation (pp. 369-392)
    Dewhurst, Frank
32. Transgenic Oilseed Rape: How to Assess the Risk of Outcrossing to Wild Relatives (pp. 393-404)
    Baranger, Alain; Kerlan, Marie-Claire; Chevre, Anne-Marie; Eber, Federique; Vallee, Patrick; Renard, Michel
33. Electroporation Protocols for Agrobacterium (pp. 405-412)
    Main, Garry D.; Reynolds, Stephen; Gartland, Jill S.

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