Library Science

A cutting-edge collection of readily reproducible methods for conducting research with adenoviruses, the premier and most widely used model in cell and molecular biology. The methods range from how to grow and titer adenoviruses and how to construct specific alterations in the adenovirus genome, to how to measure apoptosis induced by cells of the immune system, cytokines, and intrinsic apoptosis effectors. In addition, there are methods to study transcription and splicing with in vitro systems and for the adenovirus-mediated transformation of cells to a malignant state. Each method is written by a prominent investigator well-versed in the technique and includes a brief background discussion and tried, as well as true step-by-step instructions. Ed. Wold, William S. M

Table of Contents

1. Preparation and Titration of CsCl-Banded Adenovirus Stock (pp. 1-10)
   Tollefson, Ann E.; Hermiston, Terry W.; Wold, William S. M.
2. Construction of Mutations in the Adenovirus Early Region 3 (E3) Transcription Units (pp. 11-24)
   Hermiston, Terry W.; Tollefson, Ann E.; Wold, William S. M.
3. Isolation, Growth, and Purification of Defective Adenovirus Deletion Mutants (pp. 25-32)
   Ketner, Gary; Boyer, Julie
4. Manipulation of Early Region 4 (pp. 33-46)
   Boyer, Julie; Ketner, Gary
5. Adenovirus DNA Packaging: Construction and Analysis of Viral Mutants (pp. 47-60)
   Schmid, Susanne I.; Hearing, Patrick
6. Methods for Creating and Analyzing Adenovirus Vectors that Express Proteins that Act on the Viral Genome (pp. 61-84)
   Young, C. S. H.; Nicolas, Andrea L.; Lu, Heng; Munz, Patricia L.
7. Construction of Mouse Adenovirus Type 1 Mutants (pp. 85-104)
   Cauthen, Angela N.; Spindler, Katherine R.
8. Generation of Adenovirus-Specific Cytotoxic T Lymphocytes (pp. 105-112)
   Sparer, Tim E.; Gooding, Linda R.
9. Measurement of Macrophage-Mediated Cytolysis of Adenovirus-Infected Cells (pp. 113-120)
   Duerksen-Hughes, Penelope J.; Gooding, Linda R.
10. Measurement of Tumor Necrosis Factor (TNF) Lysis of Adenovirus-Infected Cells (pp. 121-128)
    Shisler, Joanna L.; Gooding, Linda R.
11. Role of Natural Killer (NK) Cells in Immunity to Adenoviruses (pp. 129-142)
    Routes, John M.
12. Determination of the Transforming Activities of Adenovirus Oncogenes (pp. 143-150)
    Subramanian, T.; Chinnadurai, G.
13. Cell Survival and Apoptosis Assays for E1B 19K and Bcl-2 Family Members (pp. 151-156)
    Subramanian, T.; Chinnadurai, G.
14. In Vitro Transcription: Probing the Molecular Functions of Adenovirus Regulatory Proteins (pp. 157-168)
    Loewenstein, Paul M.; Song, Chao-Zhong; Green, Maurice
15. Cell Microinjection: in Vivo Analysis of the Functional Domains of Viral Regulatory Proteins (pp. 169-194)
    Green, Maurice; Thorburn, Andrew; Loewenstein, Paul M.
16. Immunoprecipitation of E1A-Containing Protein Complexes (pp. 195-202)
    Moran, Elizabeth; Yaciuk, Peter
17. Preparation of Splicing-Competent Nuclear Extracts from Adenovirus-Infected Cells (pp. 203-216)
    Muhlemann, Oliver; Akusjarvi, Goran
18. Adenovirus Entry into Cells: A Quantitative Fluorescence Microscopy Approach (pp. 217-230)
    Greber, Urs F.; Nakano, Michel Y.; Suomalainen, Maarit
19. Simultaneous Detection of RNA and Proteins in Adenovirus-Infected Cells by Fluorescence In Situ Hybridization (FISH) and lmmunostaining (pp. 231-240)
    Bridge, Eileen
20. Characterization of the Adenovirus Fiber Protein (pp. 241-258)
    Engler, Jeffrey A.; Mulach, Barbara; Hong, Jeong Shin
21. Large-Scale Purification and Crystallization of Adenovirus Hexon (pp. 259-276)
    Rux, John J.; Pascolini, Donatelia; Burnett, Roger M.
22. Adenovirus Protease (pp. 277-282)
    Weber, Joseph M.
23. Methods for Growth and Purification of Enteric Adenovirus Type 40 (pp. 283-294)
    Mautner, Vivien
24. Transfection Complexes Generated with Adenovirus and Polyethylenimine-Condensed DNA (pp. 295-308)
    Cotten, Matt; Saltik, Mediyha; Baker, Adam
25. Phylogenetic Analysis of Adenovirus Sequences: Proof of the Necessity of Establishing a Third Genus in the Adenoviridae Family (pp. 309-340)
    Harrach, Balazs; Benko, Maria

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