29th
April
2007
A comprehensive collection of essential, time-tested recipes for successful protein fractionation and purification in any experimental circumstance. The protocols give step-by-step instructions on how to select a source for the protein of interest, how to obtain a usable initial extract, how to purify the protein from that extract using both chemical and molecular methods, and how to dry and store the purified protein. Protein Purification Protocols provides all that is needed to design and carry out a successful purification program. It helps both experienced and novice investigators to clarify and define their purification problems and then provides a comprehensive set of tools for a practical solution. Authors Doonan, Shawn
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posted in Molecular Biology |
29th
April
2007
Leading experts demonstrate how to successfully apply the fundamentals of NMR to the study of the properties of proteins. They cover not only assignment and structure determination techniques, but also the practical aspects of experimental set-up, protein production, data processing, and binding studies. Extensive treatments of paramagnetics and metalloproteins, membrane-bound proteins, and protein–ligand complexes are also included. Its focused treatment of proteins make it an indispensable study guide for chemists and biochemists, including both those with only a basic knowledge of NMR, as well as more experienced investigators.
Authors: Reid, David G.
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posted in Molecular Biology |
29th
April
2007
Leading practitioners authoritatively describe the newest and most effective spectrometric techniques for the analysis of proteins and peptides. The areas covered range from the elucidation of primary and secondary protein structure and the rapid identification of proteins using database techniques to methods for sequencing, as well as methods for the quantitative determination of peptides. Other chapters provide detailed information on the analysis of glycoproteins and glycopeptides and on the use of mass spectrometry to probe the interactions of proteins, both covalent and noncovalent.
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posted in Molecular Biology |