Library Science

The newest and most up-to-date collection, PCR Protocols offers detailed laboratory procedures for the use of polymerase chain reactions in a wide range of applications. Although specific examples and experimental systems are described, the book’s focus is on general applications that can be modified to suit a broad spectrum of research systems, thus making its scope appealing to nearly all researchers. PCR Protocols features clear, easy-to-follow descriptions of procedures and a Notes section in each chapter to provide tips, alternative suggestions, and other enhancements of the protocols. Key Topics and Techniques: basic startup procedures for PCR – PCR in detecting DNA and RNA – PCR in DNA synthesis and mutagenesis – PCR cloning of DNA – selection of primers – radioactive and nonradioactive labeling – quantitation of PCR products by HPLC – chromosome assignment – PCR mapping of HLA genes – sequencing of PCR products – genomic footprinting – quantitation of tumor gene expression – screening of phage libraries – SSP-PCR and genome walking – subcloning of PCR products. Authors: White, Bruce A. Read the rest of this entry »