Library Science

You will easily synthesize and analyze oligonucleotide conjugates by following the step-by-step protocols presented in this volume. These techniques are widely used by all molecular biologists and antisense researchers and find special application by pharmacologists working in new drug development and quality assurance assay.

Authors: Agrawal, Sudhir

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For volume 2: A broad-ranging collection of cutting-edge techniques for the study of HBV and HDV infections and for the development of therapies to treat them. In this second of two volumes, Immunology, Model Systems, and Clinical Studies, the authors focus on protocols for the study of host immune responses to infection, in vitro and in vivo models of infection, and the development of antivirals. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle underlying the technique, equipment and reagent lists, and tips on troubleshooting and avoiding known pitfalls. An accompanying first volume, Detection, Genotypes, and Characterization, contains user-friendly protocols for the identification and quantification of viral markers, the detection and impact of viral variants, and the study of the viral life cycle. For both volumes: A broad-ranging collection of core techniques for the study of HBV and HDV infections and for the development of therapies to treat them. The first volume Detection, Genotypes, and Characterization, the authors focus on readily reproducible molecular methods for the identification and quantification of viral markers, the detection and impact of viral variants, and the study of the viral life cycle. The second volume, Immunology, Model Systems, and Clinical Studies, contains user-friendly protocols for the study of host immune responses to infection, in vitro and in vivo models of infection, and the development of antivirals. Each fully tested protocol is described in step-by-step detail by an established expert in the field and includes a background introduction outlining the principle underlying the technique, equipment and reagent lists, and tips on troubleshooting and avoiding known pitfalls. The set offers both new and experienced investigators an encyclopedic collection of powerful tools for studying HBV and HDV infections, an essential resource for finding new therapies to treat chronically infected patients.

Authors: Hamatake, Robert K. ; Lau, Johnson Y. N.

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This second volume of protocols offers the most comprehensive set of modern analytical techniques available for studying the architecture and function of membranes. It features the application of biochemical, spectroscopic, and fluorimetric methods to the investigation of molecular topology, the dynamic aspects of membrane structure, and ion transport. Antibody technology, analysis of molecules involved in intracellular signaling, and receptor???ligand interactions are also covered.

Authors: Graham, John M.; Higgins, Joan A.

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DNA Sequencing Protocols provides you with the knowledge to become a sequencing expert. An all-star cast of investigators covers virtually all aspects, including developments in cycle sequencing, sequencing PCR products, sequencing lambda and cosmids, multiplex sequencing, direct blotting electrophoresis, sequencing of chemiluminescence, and automated sequencing.

Authors: Griffin, Annette M.; Griffin , Hugh G.

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This superb survey of the possible applications of physiochemical techniques to the analysis of biological macromolecules is designed for the practicing biochemist or biologist who wants to use the technique but lacks the experience. The contributors emphasize practical aspects, such as constraints on sample quantity, purity, and presentation; the problems of time and expense involved; the problems a technique is best suited to solve; and how the results may be interpreted. Among the methods examined are optical and infrared spectroscopy, microscopy, ultracentrifugation, calorimetry, X-ray and neutron scattering, and light scattering. This book will enable the reader to confidently collaborate with specialists in applying these techniques.

Authors: Jones, Christopher; Mulloy, Barbara; Thomas, Adrian H.

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Kenney and Fowell’s exceptionally useful new book, Practical Protein Chromatography, brings all the forefront methods of protein separation and purification directly to your laboratory benchtop. The book treats not only powerful analytical separations, but also the small- and large-scale preparative techniques that are currently applicable throughout biological and biomedical research. Chapters, written by experts in each of the techniques covered, treat a variety of methods usable in virtually every laboratory with a chromatographic system. Topics include: immunoaffinity chromatography and selection of antibodies – lectin affinity and dye???ligand chromatography – exploiting weak affinities – alternative coupling chemistries – biospecific affinity elution – size-exclusion HPLC of proteins – chromatofocusing – determination of purity and yield – specialized chromatographic techniques. The established tradition of Methods in Molecular Biology is to present techniques that guarantee optimum results. Everyone doing protein chromatography today-at any level of proficiency-will find Practical Protein Chromatography an indispensable, hands-on guide to the effective use of these methodologies.

Authors: A Kenney and S. Fowell Read the rest of this entry »

Protocols in Human Molecular Genetics highlights the tremendous advances in our ability to work on the human genome that have emerged in the past few years. The latest techniques are set forth in the clear, concise, easy-to-follow format that is the hallmark of Humana’s Methods in Molecular Biology series. Nearly two-thirds of the book is devoted to describing practical procedures comprising the widest range of new methodologies in human molecular genetics, with the rest focusing on their specific experimental and clinical applications. An essential tool for everyone-whether novice or seasoned expert-involved in the rapidly growing area of human genome studies.

Practical methods for the generation and analysis of transgenic animals, covering a comprehensive range of species, from invertebrates to lower vertebrates to higher vertebrates. Chapters examine ways in which transgenic techniques can be used to address many problems in biological systems present in-depth descriptions of analytical methods, and give step-by-step techniques that can be easily performed in your lab.

Authors: Murphy, David; Carter, David A.

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Provides key information on a wide range of enzymes commonly used as tools in molecular biology, helping to minimize the time a scientist spends researching the literature to get reactions to work efficiently and allowing the nonenzymologist to design an experiment. Each chapter gives background information on the enzyme selected and those parameters important in its use, describes both the source and application of the enzyme, and provides details on the size and structure of the protein. Specific parameters essential for achieving an optimized reaction are discussed, along with exemplary practical procedures an protocols.

Authors: Burrell, Michael M.

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Highlights recent advances in immunochemical techniques and their impact on basic research and clinical medicine, combining the forces of experts in a wide range of subspecialties to cover a wealth of information.

Authors: Manson, Margaret M. Read the rest of this entry »

Murray’s new handbook on Gene Transfer and Expression Protocols sets forth both current and new methodologies in a clear, concise, easy-to-follow manner, following the successful formula of the classic volumes in Humana’s Methods in Molecular Biology series. Each chapter is devoted to a thorough exposition of a single technique. An Introduction explains the significance of the protocol and provides background information. A Materials section lists all the requirements for the technique discussed. A Methods section details the procedure in a step-by-step protocol. A Notes section alerts the reader to pitfalls that may be encountered, as well as alternatives that may be used for successful completion of the experiment. Each technique is designed to guarantee optimum results. This volume is an outstanding new benchtop manual that provides protocols for introducing an isolated gene into a cell line, using various transfection techniques. Topics and techniques include: viral vectors – reporter genes – analysis of steady-state level of transcription – assay for newly initiated transcriptional complexes – immunocytological techniques – assay of structure and replication state of transfected genes – overall strategy for defining regulatory sequences Providing the latest techniques in a fast-paced area, Gene Transfer and Expression Protocols is an absolutely essential handbook for everyone involved in cytogenetics, cell genetics, molecular biology, and related fields.

Authors: Murray, Edward J. Read the rest of this entry »